SML4360

Aminophenyl-fluorescein

≥98% (HPLC)

• Synonim(y): 3′-p-(Aminophenyl) fluorescein, 2-[6-(4′-Amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, 3′-(4-Aminophenoxy)-6′-hydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, APF, APF, Fluorescent probe
• Wzór empiryczny (zapis Hilla): C₂₆H₁₇NO₅
• Numer CAS: 359010-70-1
• Masa cząsteczkowa: 423.42
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder
• Storage condition: desiccated

Właściwości

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated
• color: light yellow to orange
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Opis

Biochem/physiol Actions

Aminophenyl-fluorescein (APF), a cell-permeable and photostable probe, is designed for the selective detection of highly reactive oxygen and nitrogen species (hROS & hRNS), notably hypochlorite (OClˉ), hydroxyl radicals (HO•), and peroxynitrite (ONOOˉ) over minimally reactive H2O2, O2ˉ•, NO, 1O2 and ROO•. Initially exhibiting weak fluorescence (ε455 = 24,000 M-1cm-1, Φ: 0.008), APF undergoes O-dearylation upon reaction with these species, releasing its amino-phenoxy group and generating intensely fluorescent fluorescein, which leads to a dose-dependent fluorescence signal increase (λex/λem: 490/515 nm) and significantly enhanced optical properties (ε492 = 84,000 M-1cm-1, Φ: 0.85). APF′s inherent selectivity, complete autoxidation resistance, low toxicity, and quantitative fluorescence response establish it as a valuable tool for applications spanning microscopy, enzymatic assays, and multiplexing.

Disclaimer

Hygroscopic, store with desiccant

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H319
• Ostrzeżenia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable