STRN250

Spectrum^™ Plant Total RNA Kit

sufficient for 250 purifications

• Synonim(y): Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Właściwości

• usage: sufficient for 250 purifications
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Sustainability features
• technique(s): RNA purification: suitable
• test parameters: : ~30 min kit run time, sample volume: 100 mg
• storage temp.: 15-25°C

Opis

General description

Click here to view the kit protocol

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum^™Plant Total RNA Kit has been used:

• to extract RNA from the material ground in liquid nitrogen
• to isolate total RNA from mutant and wt (wild type) plantlets
• to extract total RNA from leaf samples
• to extract RNA from all plant tissues

Features and Benefits

• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

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Tylko elementy zestawu

• Binding Solution
• Lysis Solution
• Wash Solution 1

Elementy zestawu są też dostępne oddzielnie

• M3148

  2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2

  Karta charakterystyki

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08,GHS05,GHS09
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Ostrzeżenia: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Narządy docelowe: Liver,Heart
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Co się dzieje?: WGK 3