Data is not available to indicate whether DNA contamination could affect sequencing. However, including DNase treatment is recommended to minimize genomic DNA in the sequencing reads.
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STRN250
Spectrum™ Plant Total RNA Kit
sufficient for 250 purifications
Cechy związane ze zrównoważonym rozwojem
Synonim(y):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 8410,00 zł |
Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
8410,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócusage
sufficient for 250 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
storage temp.
15-25°C
General description
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry.
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
Application
Spectrum™Plant Total RNA Kit has been used:
- to extract RNA from the material ground in liquid nitrogen
- to isolate total RNA from mutant and wt (wild type) plantlets
- to extract total RNA from leaf samples
- to extract RNA from all plant tissues
Features and Benefits
- Yields up to 60 μg of pure concentrated RNA per prep
- Efficient protocol allows for RNA purification in 30 min or less
- Specially designed for research with difficult plant tissues
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description sufficient for 250 purifications | description sufficient for 50 purifications | description sufficient for 10 purifications | description sufficient for 100 extractions, sufficient for 100 amplifications |
| usage sufficient for 250 purifications | usage sufficient for 50 purifications | usage sufficient for 10 purifications | usage sufficient for 100 extractions, sufficient for 100 reactions, sufficient for 100 amplifications |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) PCR: suitable, whole genome amplification: suitable |
| test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters sample volume: 100 mg, : ~30 min kit run time | test parameters sample volume: 100 mg, : ~30 min kit run time | test parameters - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. - |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category - |
| sustainability Sustainability features | sustainability Sustainability features | sustainability Sustainability features | sustainability - |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Narządy docelowe
Liver,Heart
Klasa składowania
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
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I’m using your plant RNA extraction kit for RNA-seq. If I skip the DNase treatment, could DNA contamination affect the sequencing library, or is it mandatory to include this step? Thank you.
1 answer-
Helpful?
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does it sufficient for small RNA and/or non-coding RNA extraction?
1 answer-
Yes, this kit is sufficient for small RNA. However, It is worth noting that small RNA molecules, such as microRNA, siRNA, tRNA, 5S rRNA, are not efficiently recovered using the standard procedure. If desired, these small RNA molecules can be recovered by increasing the amount of binding solution, as described in Protocol A under Procedure Step 4.
For more information, please see the protocol associated with this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/323/177/strn250bul.pdfHelpful?
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