As listed in the 'DESCRIPTION' section under 'Preparation Note', this product is a 25 g/L porcine trypsin solution in Hank's Balanced Salt Solution with phenol red. This product does not contain EDTA.
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T4674
Trypsin solution from porcine pancreas
sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 206,00 zł |
Informacje o tej pozycji
Biological source:
Porcine pancreas
206,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Porcine pancreas
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
technique(s)
cell culture | mammalian: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.6
shipped in
dry ice
storage temp.
−20°C
Application
For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Biochem/physiol Actions
Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Preparation Note
This is a 25 g/L porcine trypsin solution in Hank′s Balanced Salt Solution with phenol red.
Other Notes
One BAEE unit will produce a A253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as a substrate.
Disclaimer
This product should be stored frozen at -20°C.
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Ta pozycja | |||
|---|---|---|---|
| description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red | description lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid | description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride | description 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source Porcine pancreas | biological source Porcine pancreas | biological source Porcine | biological source Porcine pancreas |
| form solution | form lyophilized powder | form solution | form solution |
| mol wt 23.4 kDa | mol wt 23.8 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| pH 7.6 | pH - | pH 7.6 | pH 7.0-7.6 |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
12 - Non Combustible Liquids
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9002-07-7
CAS No.
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