The amount of material in a given package size varies from lot to lot. The minimum activity of this product is 1.5 units per milligram of protein. The minimum protein concentration is 80%. As an example, a lot reporting an activity of 1.5 units per milligram with an 80% protein concentration will contain 400ug, 800ug, and 4.0 mg of powder in the 1 Unit, 2 Unit, and 10 Unit package sizes, respectively. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/t5398#product-documenation
Przejdź do
T5398
Transglutaminase from guinea pig liver
lyophilized powder, ≥1.5 units/mg protein
Synonim(y):
Protein-Glutamine-γ-Glutamyltransferase, Protein-glutamine:amine γ-glutamyltransferase
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 unit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 996,00 zł |
2 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1460,00 zł |
10 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 6790,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numer WE:
MDL number:
Specific activity:
≥1.5 units/mg protein
996,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócform
lyophilized powder
Quality Segment
specific activity
≥1.5 units/mg protein
mol wt
76.6 kDa
composition
Protein, ≥80% modified Warburg-Christian
solubility
H2O: soluble 1.0 mg/mL, clear
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Application
Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.
This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.
Biochem/physiol Actions
Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.
Physical form
Lyophilized powder containing Tris and dithioerythritol
Other Notes
One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)
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Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, ≥1.5 units/mg protein | description Glycosylation tolerant, for site-specific antibody bioconjugation | description lyophilized powder, ≥12 units/mg protein | description affinity isolated antibody |
| specific activity ≥1.5 units/mg protein | specific activity ≥30 units/mg protein | specific activity ≥12 units/mg protein | specific activity - |
| form lyophilized powder | form - | form lyophilized powder | form buffered aqueous solution |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| solubility H2O: soluble 1.0 mg/mL, clear | solubility - | solubility - | solubility - |
| storage temp. −20°C | storage temp. -10 to -25°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 76.6 kDa | mol wt - | mol wt - | mol wt antigen 77 kDa |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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Hello , how much grams are in one Unit Transglutaminase enzyme T5398 ? thanks very much kind regards Alex
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