T7513

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

• Synonim(y): Factor IIa
• Numer CAS: 9002-04-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-648-7
• MDL number: MFCD00166053
• Numer WE: 3.4.21.5 (BRENDA, IUBMB)
• Specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Właściwości

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
• mol wt: heavy chain ~33 kDa, light chain ~5 kDa
• UniProt accession no.: P00735
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: cow ... F2(280685)

Opis

Application

Thrombin from bovine plasma has been used to convert fibrinogen to fibrin for preparation of fibrin hydrogel scaffolds in tissue engineering and 3D cell culture applications.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H315,H319,H334,H335
• Ostrzeżenia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable