The recommended temperature range for products stored at -20°C is -15 to -25°C. However, this product ships globally at ambient temperatures, year-round. While stability studies at -10°C have not been performed, short excursions outside of the recommended range are not expected to be detrimental to the product quality.
Przejdź do
T8885
Thrombin from human plasma
lyophilized powder, Suitable for routine use in the thrombin time test
Synonim(y):
Factor IIa
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
10 vials | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1050,00 zł |
25 vials | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2220,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
Numer WE:
eCl@ss:
32160703
Biological source:
human plasma
1050,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócGeneral description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study that compared efficacy and safety of low-molecular-weight heparins (LMWHs) to other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery.
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Preparation Note
When reconstituted with 1 ml H2O, one vial will contain 10 mg bovine serum albumin, 0.15 M NaCl and 0.05 M sodium citrate, pH 6.5
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder, Suitable for routine use in the thrombin time test | description lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3) | description lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), suitable for cell culture | description lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3) |
| Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) | Gene Information human ... F2(2147) |
| biological source human plasma | biological source - | biological source human plasma | biological source human plasma |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 200 |
Still not finding the right product?
Explore all of our products under Thrombin from human plasma
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
11 - Combustible Solids
WGK
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
What is the stability of the stored lyophilized powder after a short period of temperature above -20°C? (Due to a technical failure of the freezer, its temperature reached -10°C)
1 answer-
Helpful?
-
-
What is the stability of this material after Lyophilization? Can I save it in the -20^0C?
1 answer-
This product is provided as a lyophilized powder. When stored appropriately at -20ºC, the material is quite stable and not assigned an expiration or retest date. A warranty period of one year from the date of shipment applies. Following reconstitution, stock solutions remain active for one week at 2 – 8ºC. Solutions are most stable at pH 6.5, as a pH >7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot solutions in plastic tubes and store at –20 ºC for long-term storage. Various literature sources indicate solutions may be most stable when stored at -70ºC. The long term stability of this product in solution has not been established.
Helpful?
-