U7752

Urease from Canavalia ensiformis (Jack bean)

• Numer CAS: 9002-13-5
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-656-0
• NACRES: NA.54
• MDL number: MFCD00070858
• Numer WE: 3.5.1.5 (BRENDA, IUBMB)
• Biological source: Canavalia ensiformis

Właściwości

• biological source: Canavalia ensiformis
• Quality Segment: 200
• form: solid
• mol wt: hexamer 545 kDa, trimer ~272 kDa
• contains: ~20% dithiothreitol
• suitability: suitable for marker for electrophoresis (non-denaturing PAGE)
• storage temp.: −20°C

Opis

General description

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

• as a component of the molecular mass standard in electrophoresis
• as a component of the standard in size-exclusion chromatography
• as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Other Notes

Contains approx. 1 mg protein

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H315,H319,H334,H335
• Ostrzeżenia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 1