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Merck

Soy Preparations Are Potentially Dangerous Factors in the Course of a Food Allergy.

Foods (Basel, Switzerland) (2019-12-11)
Anna Jędrusek-Golińska, Dorota Piasecka-Kwiatkowska, Paulina Zielińska, Magdalena Zielińska-Dawidziak, Krystyna Szymandera-Buszka, Marzanna Hęś
ABSTRAKT

The special properties of soy preparations make them common additives for food production and can be dangerous for sensitive individuals. Our aim was to check consumers' awareness of potential risks of soy preparations added to numerous food products, depending on respondents' education, and to evaluate immunoreactive properties of chosen soy preparations. A personal questionnaire was used. Respondents (n = 251) were aged 23-28 years old, lived in Poland, and were graduates or students in their last year of food technology, medicine, and university of technology. The slot blot and Western blotting methods were used to mark immunoreactivity of soy preparations. It was shown that most respondents often or usually read labels of food products they buy. The surveyed indicated protein is the allergenic component in soy. Almost half of them were of the opinion that hydrolysis removes the allergenic properties of soy. Most of the medical students surveyed thought that people allergic to soy may consume products that contain soy preparations. The analytical results indicated that soy preparation contained protein fractions that were immunoreactive with sera of allergenic patients. It was proven that preparations, even hydrolysates, contain immunoreactive proteins that may be the source of hidden allergens, even though they are not recognized as dangerous by well-educated respondents.

MATERIAŁY
Numer produktu
Marka
Opis produktu

Sigma-Aldrich
MonoklonalnaIgG przeciw królikowi(specyficzna dla łańcucha γ)-fosfataza alkaliczna, clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Soy Protein antibody produced in rabbit, fractionated antiserum, buffered aqueous solution
Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, pH 7, ≥98%
Sigma-Aldrich
TWEEN® 20, Molecular Biology, viscous liquid
Millipore
Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Sigma-Aldrich
Przeciwciało przeciw ludzkiej IgE-fosfatazie alkalicznej, mysie monoklonalne, clone GE-1, purified from hybridoma cell culture