- Labeling of monoclonal antibodies with 153Sm for potential use in radioimmunotherapy.
Labeling of monoclonal antibodies with 153Sm for potential use in radioimmunotherapy.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine (2002-11-16)
M Fani, S Vranjes, S C Archimandritis, S Potamianos, S Xanthopoulos, P Bouziotis, A D Varvarigou
PMID12433040
ABSTRACT
The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with 153Sm has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs.
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