03-0196-00

SMC^® Human Total α-synuclein High Sensitivity Immunoassay Kit

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

• 别名: Human α-synuclein, α-synuclein, Human total-α-synuclein, a-synuclein, total-a-synuclein
• 分類程式碼代碼: 12161503
• NACRES: NA.84

属性

• 描述: 0.49-3.91pg/mL, refer to CoA for lot-specific LLOQ
• 物種活性: canine, rat, feline, mouse
• 製造商／商標名: SMC^®
• assay range: sensitivity: 1.95 pg/mL; (LLOQ of Total α-synuclein); inter-assay cv: 14%; intra-assay cv: 9%; (five normal plasma samples run in triplicate by 3 different operators on 3 different days)
• 技術: single molecule counting: suitable

说明

一般說明

The SMC^® Human Total-α-Synuclein High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

應用

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

生化／生理作用

Species Reactivity Note

Specificity to the following species samples were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 100%
Beagle – 100%

Cross Reactivity

Cross-reactivity to the following analytes were tested with the following results:
Tau – 0%UCHL1 – 0%
β-synuclein – 0%
γ-synuclein – 0%

Accuracy

CSF Recovery: 5 CSF samples spiked with average recovery of 96% and a range of 90-102%
Serum Recovery: 4 serum samples spiked with average recovery of 96% and a range of 93-98%
Plasma Recovery: 4 plasma samples spiked with average recovery of 96% and a range of 93-97%

包裝

96 well assay

其他說明

• Assay Buffer
• Total α-synuclein Coated Beads
• Standard Diluent
• Total α-synuclein Detection Antibody
• Total α-synuclein Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

• This new streamline kit has the following features:
• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Able to achieve assay specifications on both SMCxPRO^® and Erenna.

法律資訊

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS06,GHS08
• 訊號詞: Danger
• 危險聲明: H302 + H332,H311,H319,H360FD,H412
• 防範說明: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• 危險分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• 儲存類別代碼: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable