Yes, the Cell Signaling Lysis Buffer (43-040) can be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K). The buffer was tested at dilutions of 1:2, 1:5, and 1:10 in Assay Buffer, with the 1:5 dilution showing comparable results to the Assay Buffer for most cytokines and the 1:10 dilution providing optimal results if the samples can tolerate that level of dilution.
MCYTMAG-70K-PX32
MILLIPLEX®小鼠细胞因子/趋化因子磁珠试剂盒 - 预混32重 - 免疫学多重分析
Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay
1 of 2 reviewers received a sample product or took part in a promotion
别名:
Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Assay
跳至
品質等級
物種活性
mouse
製造商／商標名
Milliplex®
assay range
accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL
技術
multiplexing: suitable
相容性
configured for Premixed
檢測方法
fluorometric (Luminex xMAP)
運輸包裝
wet ice
一般說明
免疫系统是由生物结构和过程组成的有组织的复杂网络，可预防疾病。例如，细胞因子和趋化因子直接介导细胞之间的相互作用，调节靶免疫细胞反应。这些反应导致炎症，免疫系统试图根除外来抗原并开始愈合过程。因此，炎症过程在免疫中起关键的保护作用。此外，细胞因子和趋化因子研究对于理解免疫系统及其对大多数抗原的多方面反应以及疾病状态（例如自身免疫性疾病、过敏反应、败血症和癌症）至关重要。
为了鉴别参与任何炎症或免疫反应的特定细胞因子，可能有必要筛选细胞因子组，通常需要一定水平的自动化和/或高通量。微珠可以使自动化和高通量筛选过程更容易，并具有自动化洗涤等功能。甚至在自动化之外的优势包括:
更柔韧的板和洗板机选择
MILLIPLEX®小鼠细胞因子/趋化因子组套使您能够专注于细胞因子的治疗潜力以及细胞因子表达的调节。结合微珠格式的Luminex ® xMAP®平台，您可以获得理想的速度和灵敏度的优势，允许同时对数十种分析物进行定量多重检测，从而可以显著提高生产力。
组套类型：细胞因子/趋化因子
为了鉴别参与任何炎症或免疫反应的特定细胞因子，可能有必要筛选细胞因子组，通常需要一定水平的自动化和/或高通量。微珠可以使自动化和高通量筛选过程更容易，并具有自动化洗涤等功能。甚至在自动化之外的优势包括:
更柔韧的板和洗板机选择
- 使用浑浊的血清/血浆样品改善性能
- 测定结果等同于非微珠
- 自动洗涤避免了与真空过滤洗涤相关的许多问题
MILLIPLEX®小鼠细胞因子/趋化因子组套使您能够专注于细胞因子的治疗潜力以及细胞因子表达的调节。结合微珠格式的Luminex ® xMAP®平台，您可以获得理想的速度和灵敏度的优势，允许同时对数十种分析物进行定量多重检测，从而可以显著提高生产力。
组套类型：细胞因子/趋化因子
應用
- 分析物：Eotaxin/CCL11、G-CSF、GM-CSF、IFN-γ、IL-1α、IL-1β、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-9、IL-10、IL-12 (p40)、IL-12 (p70)、IL-13、IL-15、IL-17、IP-10、KC、LIF、LIX、MCP-1、M-CSF、MIG、MIP-1α、MIP-1β、MIP-2、RANTES、TNF-α、VEGF
- 建议的样品类型：血浆、血清或细胞培养上清液
- 建议的样品稀释度：1:2稀释的血浆或血清
- 测定运行时间：一夜或一天。为了获得最佳结果，建议过夜孵育。
- 研究类别：炎症&免疫学
生化／生理作用
交叉反应性
抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。
抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。
包裝
96孔板
方便的预混珠。
準備報告
试剂盒组件的建议储存温度为2 - 8°C。
其他說明
灵敏度：有关单个细胞因子的灵敏度，请参阅试剂盒方案。
法律資訊
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
訊號詞
Danger
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
標靶器官
Respiratory Tract
儲存類別代碼
10 - Combustible liquids
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Can the Cell Signaling Lysis Buffer (43-040) be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K)?
1 answer-
Helpful?
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How do I enter the high, low, and expected concentrations for the kit QCs into Xponent? Since the QC range sheet only includes the high and low ranges, could you please provide the expected concentration as well?
1 answer-
A quality control range sheet is available, providing different values for quality controls. The expected average per range can be calculated by adding the high and low numbers for each marker, then dividing the total by 2.
Helpful?
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