Microcon and Amicon Ultra products are very similar. Both concentrate liquids down to a retentate and a filtrate. Substances bigger than the molecular weight cutoff stay on top of the membrane and are reduced from a larger initial volume to a smaller final volume. Two key differences are: the Microcon can be slightly better to use for substances in lower initial concentrations and when substances are sticky. Higher recovery can occur with Microcon due to the product being physically smaller and thus, less product is lost to the hold up volume of the membrane and plastic sides of the tube.
材料
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
品質等級
無菌
non-sterile
特點
holdup volume> 10 μL
包裝
pkg of 100 ea
製造商／商標名
Microcon®
參數
0.5 mL sample volume
技術
DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable
長度
4.50 cm (1.8 in.)
直徑
12.3 mm
過濾面積
0.32 cm2
有效容積
0.5 mL
孔徑
10 kDa NMWCO
運輸包裝
ambient
一般說明
使用任何可以容纳1.5 mL离心管的离心机，简单而高效地对任何大分子溶液进行浓缩和除盐。带有低结合性Ultracel滤膜的Microcon滤器可提供：
•通常大于95%的回收率，即便是对于稀溶液
•反向旋转以获取最高的回收率，即便是在最小的样品中
• 可将滤液或浓缩样品方便地储存在标准微量离心管中
•浓缩因子最高达100X
特点：
•通过反向旋转实现小体积的高回收率（浓缩因子低于20X）
•低结合性Ultracel滤膜
•快速处理
•通常大于95%的回收率，即便是对于稀溶液
•反向旋转以获取最高的回收率，即便是在最小的样品中
• 可将滤液或浓缩样品方便地储存在标准微量离心管中
•浓缩因子最高达100X
特点：
•通过反向旋转实现小体积的高回收率（浓缩因子低于20X）
•低结合性Ultracel滤膜
•快速处理
滤器类型：超滤
滤器编码：PL，超低®结合再生纤维素
應用
这款0.5 mL离心式过滤器借助10 kDa NMWL的滤膜可以进行生物分子浓缩。Micron离心式过滤器是DNA&蛋白浓缩&脱盐的理想工具。
特點和優勢
最小最终浓缩体积：50 µL
外觀
色码：绿色
其他說明
替代品：42407
法律資訊
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Lilly Toker et al.
Chemico-biological interactions, 319, 109007-109007 (2020-02-23)
Acetylcholinesterase (AChE) terminates cholinergic neurotransmission by hydrolyzing acetylcholine. The collagen-tailed AChE tetramer is a product of 2 genes, ACHE and ColQ. The AChE tetramer consists of 4 identical AChE subunits and one polyproline-rich peptide, whose function is to hold the
Ruth Fair-Mäkelä et al.
Mucosal immunology, 13(2), 245-256 (2019-11-28)
All leukocytes can get entrance into the draining lymph nodes via the afferent lymphatics but only lymphoid cells can leave the nodes. The molecular mechanisms behind this phenomenon have remained unknown. We employed genome wide microarray analyses of the subcapsular
Anthony O Beas et al.
Nature communications, 11(1), 2790-2790 (2020-06-05)
Age-dependent changes in metabolism can manifest as cellular lipid accumulation, but how this accumulation is regulated or impacts longevity is poorly understood. We find that Saccharomyces cerevisiae accumulate lipid droplets (LDs) during aging. We also find that over-expressing BNA2, the
Yaowei Liu et al.
Food chemistry, 314, 126177-126177 (2020-01-20)
This study mainly investigated changes in milk serum proteins by simulating the industrial processing of whole milk powder using a label-free proteomic approach. A total of 391 proteins were identified, 89 of which were quantified in all groups. Compared with
Gulzhan Trimova et al.
Arthritis research & therapy, 22(1), 24-24 (2020-02-14)
14-3-3η is an intracellular protein also detected in the serum and synovial fluid of patients with rheumatoid arthritis (RA). It is closely related to disease activity and anti-cyclic citrullinated peptide antibody levels. However, the main source of 14-3-3η and the
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