Yes, both MSIPS4W10 and S5EM077I10 correspond to the same plate, identified by the description: MultiScreen HTS IP PVDF 0.45um White Sterile 10pk. MSIPS4W10 is the public catalog number, whereas S5EM077I10 is a custom order request for a specific customer.
材料
Durapore PVDF membrane
flat bottom wells
white acrylic plate
品質等級
描述
Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.
無菌
sterile
用途
Automated
特點
hydrophobic
lid
包裝
pkg of 10 plates
製造商／商標名
MultiScreen®
技術
ELISPOT: suitable
cell based assay: suitable
dot blot: suitable
過濾面積
0.28 cm2
板尺寸
96 wells
孔最大容積
300 μL
有效容積
50-250 μL
基質
Immobilon®-P
孔徑
0.45 μm pore size
結合類型
high binding surface
相容性
centrifuge & manifold
運輸包裝
ambient
一般說明
聚偏氟乙烯(PVDF)滤膜板胜过混合纤维素酯膜，对捕获抗体的结合能力略有提高，斑点检测性能优异，尤为适合荧光法应用。
應用
法律資訊
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
实验方案
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
相关内容
MultiScreen® Filter Plates
"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
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Do MSIPS4W10 and S5EM077I10 refer to the same plate?
1 answer-
Helpful?
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Active Filters
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Great plate for ELISPOTS
These plates give a strong ELISPOT signal with very low background and work well with either a vacuum manifold plate washing apparatus or manual washing.
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