Yes, extending the spin times is sufficient. However, the time range would have to be experimentally determined by the user.
产品名称
Amicon® Ultra过滤器,10 kDa MWCO, sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL
4 mL
pore size
10 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
General description
垂直设计和有效的膜表面积提供了快速的样品处理,高样品回收率(通常大于90%的稀起始溶液),以及80倍浓度的处理能力。垂直设计最大限度地减少了溶质极化和随后的膜污染,过滤装置中的物理永停点可防止纺丝干燥和潜在的样品损失。
使用移液管从过滤装置的样品库中收集浓缩液,同时将超滤液收集在所提供的离心管中。该装置可在摆动斗中旋转,也可在定角转子中旋转。Amicon® Ultra 15 mL过滤器采用垂直膜设计和永停点,可在短旋转时间内提供最高回收率。
Application
- 采用10,000 Dalton分子量截止超滤®膜进行超滤。
- 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
- 纯化在组织培养提取物或细胞裂解液中发现的大分子成分,并在HPLC分析之前去除蛋白质。
- 从柱洗脱液稀释或预先纯化的蛋白质的浓度。
- 脱盐,缓冲液更换和蛋白质透析。
- 体积排阻和蛋白质分离。
Features and Benefits
- 使用10 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率。
- 垂直膜减少了超快旋转时间(快至5分钟)的浓度极化。
- 热封膜减少了下游的可提取物。
- 100%完整性测试,确保可靠性能。
- 半透明外壳和体积刻度便于样品监测。
- 直接移液管进样,省去了回收浓缩液的处理步骤。
- 80–100X高浓缩系数。
Legal Information
存储类别
10-13 - German Storage Class 10 to 13
商品
纳米颗粒纯化和生产中过滤方法和滤膜的选择。
Filtration methods and membrane filter selection in nanoparticle purification and production.
实验方案
人血浆蛋白质组是生物标志物发现和研究的广阔资源。
准确测定尿液蛋白对于疾病诊断和管理至关重要。
有了Amicon® Ultra标准式离心超滤管,不再需要对样品进行稀释,只要经过几轮洗滤操作,就能有效去除抗体标记反应中的未结合标记物。
生物标志物在药物发现和开发中起关键作用,
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If my centrifuge only goes to 3,052 g, is it okay to centrifuge for a longer period of time to get the same separation? It is a swing-bucket centrifuge using the 10 MWCO filters.
1 answer-
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is-it possible to wash and re-use the Amicon@Ultra-4 ? if yes how many time is_it possible to use them ?
1 answer-
The unit is for one-time use only.
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How should the tubes be sterilized without damaging the membrane?
1 answer-
There are no validated sterilization methods for the Amicon Ultra devices. However, these devices can be "sanitized" with a spin cycle with 70% ethanol, followed by 2 rinsing spins with sterile water. It is recommended to filter the concentrated sample using a sterile 0.2 µm syringe filter (e.g., SLGVR33RS) after processing on the Amicons.
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What speed can this product be centrifuged at ?
1 answer-
For this item when using a swinging-bucket rotor, spin the device at 4,000 × g maximum for approximately 10-40 minutes. When using a fixed-angle rotor, orient the device with the membrane panel facing up. For Amicon® Ultra 3K, 10K, 30K, and 50K devices, spin at 7,500 × g maximum for approximately 10-40 minutes. For Amicon® Ultra 100K devices, spin at 5,000 × g maximum for approximately 10-20 minutes.
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How to use it...how much sample should be loaded and what will be the protocol of centrifugation while using?
1 answer-
The maximum initial sample 4 volume (mL). For more information please see page 36 in this product https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
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Active Filters
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