polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
non-sterile
Amicon®
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
Amicon® Ultra
Greener Alternative Product
15 mL sample volume
protein extraction: suitable
protein purification: suitable
12.1 cm (4.807 in.)
2.97 cm
7.6 cm2
15 mL
≤15 mL
15 mL
10 kDa MWCO
ambient
本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
纳米颗粒纯化和生产中过滤方法和滤膜的选择。
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。
The spin conditions for a swinging-bucker rotor are 4000 x g and for a fixed-angle rotor are 5000 x g, at a time range of 10 - 60 minutes, based on molecular weight cut-off.
Please see the link below to review the Product User Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/181/pr05035wrev1023-ms.pdf
Helpful?
No data is available on the maximum or minimum temperature for using the Amicon Ultra-15. Results shown in the user guides are based on experiments conducted at approximately 25°C, not with refrigerated centrifuges. Some users work at 4°C, but filtration times are longer. No studies have been performed at 0°C. Glycerol is compatible with this product.
Helpful?
Concentrating cell-propagated respiratory syncytial virus stock with this product has not been studied. However, it can be attempted, as other viruses such as SARS, AAV, and Lenti have been successfully concentrated. The product concentrates substances larger than the molecular weight cut-off of the membrane. It is recommended to consult online research articles using keywords like "Amicon and respiratory syncytial virus" on platforms such as PubMed or Google Scholar.
Helpful?
Yes, product UFC5010, the Amicon Ultra 2.0 for samples of 0.5 - 2.0 mL, will accommodate a 1.5 mL sample size. Please see the link below to review this product option:
https://www.sigmaaldrich.com/product/mm/ufc5010
Helpful?
Does ultrafiltration of protein samples result in lower migration on an SDS-PAGE gel?
Yes, ultrafiltration of protein samples can potentially lead to lower migration on an SDS-PAGE gel.During the ultrafiltration process, the desalting or buffer exchange step may remove or reduce sodium chloride (NaCl), leading to lower protein migration on the gel. To prepare protein samples for gel electrophoresis, using an Amicon Ultra (AU) device or a suitable desalting/buffer exchange method is recommended.
Helpful?
Amicon® Ultra-15 Centrifugal Filter Unit comes in 8, 24, and 96/pk. the pack size is indicated in the last two digits of the catalog numbers.
Helpful?
This product is suitable for sample volumes of less than 15 mL. Please see the link below to review the estimated volumes:
https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#howtoselect
Helpful?
We do not recommend reusing the device because eventually the membrane will clog completely. We have yet to record an efficient way to clean the pores of the membrane without damaging the membrane itself.
Helpful?
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