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Merck

UFC9030

Amicon® Ultra离心超滤管，30 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa

Amicon® Ultra Centrifugal Filter, 30 kDa MWCO

别名:

15 mL样品体积, 30 kDa MWCO, 离心浓缩器

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关于此项目

分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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材料

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

品質等級

400

無菌

non-sterile

產品線

Amicon®

包裝

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

製造商／商標名

Amicon® Ultra

sustainability

Greener Alternative Product

參數

15 mL sample volume

技術

protein extraction: suitable
protein purification: suitable

長度

12.1 cm (4.807 in.)

直徑

2.97 cm

過濾面積

7.6 cm2

容積

15 mL

有效容積

≤15 mL
15 mL

孔徑

30 kDa MWCO

環保替代類別

Re-engineered

運輸包裝

ambient

相关类别

一般說明

Amicon® Ultra 15 mL离心装置与Ultracel® 30000 Dalton分子量截止滤波器提供快速超滤，以及可靠的大分子保留。例如，1 mg/ml初始浓度下的卵白蛋白(45 kDa)保留率为>95%。

垂直设计和有效的膜表面积提供了快速的样品处理，高样品回收率（通常大于90%的稀起始溶液），以及80倍浓度的处理能力。垂直设计最大限度地减少了溶质极化和随后的膜污染，过滤装置中的物理永停点可防止纺丝干燥和潜在的样品损失。

使用移液器从过滤装置的样品库中收集浓缩液，同时将超滤液收集在所提供的离心管中。该装置可在摆动斗中旋转，也可在定角转子中旋转。Amicon® Ultra 15 mL过滤器采用垂直膜设计和永停点，可在短旋转时间内提供最高回收率。
我们竭诚为您带来经过重新设计的绿色替代产品，实现可持续性更高的研究方案。 内部过滤装置采用按ISCC质量平衡分配的60%生物循环材料。点击此处以获取更多信息。

應用

  • 采用30,000 Dalton分子量截止超滤®膜进行超滤。
  • 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
  • 纯化在组织培养提取物或细胞裂解液中发现的大分子成分，并在高效液相色谱法之前去除蛋白质。
  • 从柱洗脱液稀释或预先纯化的蛋白质的浓度。
  • 脱盐，缓冲液更换和蛋白质透析。

特點和優勢

  • 使用30 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率。
  • 垂直膜减少了超快旋转时间（快至10 - 15分钟）的浓度极化。
  • 热封膜减少了下游的可提取物。
  • 100%完整性测试，确保可靠性能。
  • 半透明外壳和体积刻度便于样品监测。
  • 直接移液器进样，省去了回收浓缩液的处理步骤。
  • 80–100X高浓缩系数。

外觀

色码：透明，带有粉色盖

法律資訊

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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商品

纳米颗粒超滤

纳米颗粒纯化和生产中过滤方法和滤膜的选择。

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

蛋白样品超滤

本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。

实验方案

Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

使用 Amicon® Ultra 离心超滤管快速浓缩抗体

免疫球蛋白纯化方案通常包括与Protein A或Protein G层析介质的亲和吸附、未吸附蛋白洗涤以及低pH缓冲液洗脱。

相关内容

Sample Purification

Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.

样品纯化

下游分析所用的纯化方法旨在降低样品复杂程度、去除干扰并浓缩分析物，确保获得更准确的结果。

Amicon® Ultra离心超滤管

浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。

User Guide-Amicon® Ultra-15 Centrifugal Filter Devices
2 Reviews This action will navigate to reviews.

Questions

1–8 of 8 Questions  

  1. how many units are there in 1 pkg

    1 answer

    1. This product is packaged as a box of 8 tubes, 24 tubes, or 96 tubes.

      Helpful?

  2. How many individual columns come in one package. For example, if I wanted to purchase the 24package option, wil I get 24 columns or 24 packages of x number of columns?

    1 answer

    1. A package of 24 would equate to 24 Ultra Centrifugal Filters.

      Helpful?

  3. Hi there, I need to extract exosome in the range of 30 to 150 nm. Which Ultra Centrifugal Filter is fit for my work? Thank you for your advice in advance.

    1 answer

    1. We have performed studies of nanometer particles and all of our studies show that using a 100 kda molecular weight cutoff should be fine to trap particles greater than 10 nm.  

      Helpful?

  4. Can this item be sterilised, and if so, what is the best method (e.g., autoclaving) to ensure that product function and integrity are maintained?

    1 answer

    1. This item cannot be autoclaved. Sterilization with Ethylene Oxide is possible, but the method has not been validated.

      Helpful?

  5. Can the product be washed and re-used?

    1 answer

    1. This product is intended for single use. Repeated usage may result in declining performance.

      Helpful?

  6. How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.

    1 answer

    1. The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.

      Helpful?

  7. I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?

    1 answer

    1. The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.

      Helpful?

  8. Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

2 Reviews This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. San Diego
    • Review 1
    • Votes 10
    5 out of 5 stars.

    Amicon Ultra-15-An excellent choice

    This was back ordered a long time, but it is an excellent concentrator.

    Helpful?

  2. 1 Ratings-only review

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