This kit is used for the detection of hippurate hydrolase, an enzyme produced by a number of pathogenic organisms. Hippurate is the substrate embedded into the test strips. Hippurate hydrolase produced by bacteria will hydrolyze the embedded hippurate in the test strip. One of the resulting by-products, glycine, reacts with the chromogen producing a blue/purple color, indicating a positive test. This is a qualitative test. Tests are limited to cultures taken from samples that have been incubated for at least 24 hours. See the link below to review the protocol: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/398/494/01869dat.pdf
技術
microbe id | specific enzyme detection: suitable
品質等級
應用
clinical testing
environmental
food and beverages
microbiology
儲存溫度
2-8°C
適合性
Campylobacter spp.
Gardnerella vaginalis
Streptococcus spp.
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應用
本试验的原理是底物(马尿酸钠)被细菌酶马尿酸盐水解酶水解并生成苯甲酸和甘氨酸。在孵育 24 小时后,通过与色原体(茚三酮)反应(生成蓝紫色物质)而检测该酶反应产生的甘氨酸。
其他說明
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system, Respiratory system
儲存類別代碼
3 - Flammable liquids
閃點(°F)
50.0 °F - closed cup
閃點(°C)
10 °C - closed cup
個人防護裝備
Eyeshields, Gloves, type N95 (US)
商品
对于微生物学家而言,最基础的染色方法是由丹麦细菌学家Hans Christian Gram在1884年开发的。
For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.
实验方案
近年来,李斯特菌病被定为重大的公共健康问题。这种病的主要感染对象是老年人、孕妇、新生儿和免疫功能低下者。
Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.
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Hello, Is this product suitable to detect if there is any hippurate in our samples? Is it a quantitative test or a qualitative test? Are there any restrictions on sample types?
1 answer-
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