The expiration date of Accuspin tubes is stated on the box, andits not possible to specify a shorter shelf life after opening. Individual Accuspin tube is sterile until the cap is removed, and the opened box or tube stability can only be determined by the user.
产品名称
ACCUSPIN™ 系统-Histopaque®-1077, sterile-filtered, density: 1.077 g/mL
sterility
aseptically filled
form
solution
shelf life
Expiry date on the label.
packaging
pkg of packaging 40 tubes
technique(s)
cell culture | mammalian: suitable
density
1.077 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
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此商品 | A7054 | 10771 | A0561 |
|---|---|---|---|
| form solution | form solution | form liquid | form solution |
| sterility aseptically filled | sterility aseptically filled | sterility sterile-filtered | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| density 1.077 g/mL | density 1.077 g/mL | density 1.077 | density 1.077 g/mL |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| packaging pkg of packaging 40 tubes | packaging pkg of package 12 tubes | packaging - | packaging package of 100 tubes |
Application
ACCUSIN System-Histopaque-1077使用密度梯度分离介质从全血和骨髓中分离淋巴细胞和其他单核细胞。Histopaque-1077用于：
- 人淋巴细胞抗原(HLA)分型
- T、B和‘裸’淋巴细胞计数前的起始分离。
- 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
- 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
- 分离人类淋巴细胞
- 分离全血中乳癌循环肿瘤细胞(CTC)
ACCUSPIN System-HISTOPAQUE-1077 采用专门设计的离心管，两个腔室由多孔高密度聚乙烯屏障（“熔块”）隔开。下部腔室含有 HISTOPAQUE-1077，可添加抗凝全血而不存在与分离介质混合的风险。离心时，全血通过玻璃料下降，与玻璃料下方的 HISTOPAQUE-1077 接触，使血液成分明显分离。红细胞聚集，粒细胞变得略微高渗，增加其沉降率，导致 ACCUSPIN 管底部出现颗粒。淋巴细胞和其他单核细胞，即单核细胞，保留在血浆-HISTOPAQUE-1077 界面。
Biochem/physiol Actions
下部分含有HISTOPAUE-1077，保证添加抗凝全血时不会与分离介质混合。离心后，全血下沉通过筛板，与筛板下方的HISTOPAQUE-1077接触，从而将血液成分完全分离。红细胞聚集，粒细胞变得略微高渗，增加其沉降率并导致ACCUSPIN管底部出现沉淀。淋巴细胞和单核白细胞等其他单核细胞留在血浆-HISTOPAQUE-1077分界面。
Features and Benefits
ACCUSPIN™ System-Histopaque®-1077具有很多优点，例如：
- 可使活细胞极佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在 2-8°C 避光保护条件下可稳定保存至少2年
General description
ACCUSIN System-Histopaque-1077产品用于分离淋巴细胞和其他单核细胞。分离介质Histopaque-1077是一种无菌过滤的、经内毒素检测的聚蔗糖和泛影酸钠溶液，密度调整为1.077 g/mL。ACCUSPIN系统-Histopaque 1077采用γ辐照的12mL聚丙烯离心管，专门设计多孔高密度聚乙烯屏障（“筛板”）分出两个腔室。每管无菌灌装3 mL的Histopaque 1077。每管可分离3-6 mL抗凝血。
Preparation Note
每包装含 40 个预装管
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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