跳转至内容
Merck

E1510

溴化乙啶 溶液

BioReagent, Molecular Biology, 10 mg/mL in H2O

别名:

3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵

登录 查看公司和协议定价

选择尺寸

10 ML

$195.00

$195.00


即日发货详情


获取大包装/定制包装报价

关于此项目

经验公式(希尔记法):
C21H20BrN3
化学文摘社编号:
分子量:
394.31
Beilstein:
3642536
MDL號碼:
分類程式碼代碼:
41105322
PubChem物質ID:
NACRES:
NA.31

跳至

技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

等級

Molecular Biology

產品線

BioReagent

濃度

10 mg/mL in H2O

技術

electrophoresis: suitable

適合性

suitable for gel electrophoresis

SMILES 字串

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI 密鑰

ZMMJGEGLRURXTF-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
E1385E763746067
technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for fluorescence

concentration

10 mg/mL in H2O

concentration

500 μg/mL in H2O

concentration

-

concentration

~1% in H2O

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

應用

溴化乙啶 (EtBr) 为聚丙烯酰胺凝胶电泳或琼脂糖凝胶电泳中最常用的核酸染料。EtBr 在结合到双链 RNA 上时其荧光可提高 21 倍,在结合到双链 DNA 上可提高 25 倍,从而使得在低染料浓度 (10μg/ml) 下无需背景脱色。溴化乙啶已用于核酸的许多荧光分析。已表明它可结合到单链 DNA(虽然强度不高)和三链 DNA 上。由于 EtBr 能够结合到 DNA 上,因此它是 DNA 聚合酶的抑制剂。
溴化乙锭溶液可用于:
  • 用作可视化U937细胞的染色剂,以评估细胞活性[1]
  • 检测聚合酶链反应产物[2]
  • 基于琼脂糖凝胶电泳的凝胶阻滞测定[3]

生化/生理作用

溴化乙啶可插入双链 DNA 和 RNA 中,并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

準備報告

对于在电泳后进行凝胶染色,应先用水将储存液样品稀释到 0.5μg/ml,再将凝胶孵育 15-30 分钟。通常无需脱色,但是如果必须降低背景色,则可在水中脱色 15 分钟。然后可在紫外灯箱(254nm 波长)中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液(终浓度 0.5μg/ml)中,并在电泳后立即显影。

同類產品

产品编号
说明
价格

相關產品

产品编号
说明
价格

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 3 Inhalation - Muta. 2

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

pH-sensitive oncolytic adenovirus hybrid targeting acidic tumor microenvironment and angiogenesis
Choi JW, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 205, 134-143 (2015)
Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells
Quinones GA, et al.
Blood, 121(7), e25-e33 (2013)
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
Patrick Seitz et al.
PLoS genetics, 10(1), e1004066-e1004066 (2014-01-07)
The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is

实验方案

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

查看所有结果

相关内容

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

No rating value

Active Filters

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持