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Merck

G4259

β-葡萄糖醛酸酶 来源于散大蜗牛 （花园蜗牛）

Type HA-4

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关于此项目

EC 号:
3.2.1.31 (BRENDA, IUBMB)
EC號碼:
232-606-8
MDL號碼:
MFCD00130629
分類程式碼代碼:
12352204
NACRES:
NA.54

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種類

Type HA-4

形狀

partially purified powder

比活性

≥300,000 units/g solid solid

secondary activity

≤7,500 units/g solid sulfatase

溶解度

H2O: soluble 1.90-2.10 mg/mL, clear to slightly hazy

應用

clinical testing

儲存溫度

−20°C

相关类别

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此商品
G0751G1512G0251
β-葡萄糖醛酸酶 来源于散大蜗牛 （花园蜗牛） Type HA-4

Sigma-Aldrich

G4259

β-葡萄糖醛酸酶 来源于散大蜗牛 （花园蜗牛）

β-葡萄糖醛酸酶 来源于罗曼蜗牛 Type H-1, partially purified powder, ≥300,000 units/g solid

Sigma-Aldrich

G0751

β-葡萄糖醛酸酶 来源于罗曼蜗牛

β-葡萄糖醛酸酶 来源于罗曼蜗牛 Type H-5, lyophilized powder, ≥400,000 units/g solid

Sigma-Aldrich

G1512

β-葡萄糖醛酸酶 来源于罗曼蜗牛

β-葡萄糖醛酸酶 来源于牛肝脏 Type B-1, ≥1,000,000 units/g solid

Sigma-Aldrich

G0251

β-葡萄糖醛酸酶 来源于牛肝脏

specific activity

≥300,000 units/g solid solid

specific activity

≥300,000 units/g solid

specific activity

≥400,000 units/g solid

specific activity

≥1,000,000 units/g solid

form

partially purified powder

form

partially purified powder

form

lyophilized powder

form

solid

application(s)

clinical testing

application(s)

-

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 1.90-2.10 mg/mL, clear to slightly hazy

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

應用

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.[1]

生化／生理作用

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption[2][3][4].

其他說明

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).
One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
098K37941
098K3794
017K3785
128H3780
128H37801

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Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>
Marathe &amp; J.E. McEwen
Gene, 154, 105-107 (1988)
Catalytic mechanisms of enymatic glycosyl transfer
M.L. Sinnott
Chemical Reviews, 90, 1171-1202 (1990)
J D McCarter et al.
Current opinion in structural biology, 4(6), 885-892 (1994-12-01)
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses
S Jain et al.
Nature structural biology, 3(4), 375-381 (1996-04-01)
The X-ray structure of the homotetrameric lysosomal acid hydrolase, human beta-glucuronidase (332,000 Mr), has been determined at 2.6 A resolution. The tetramer has approximate dihedral symmetry and each promoter consists of three structural domains with topologies similar to a jelly
Jie Xing et al.
Journal of pharmaceutical and biomedical analysis, 39(3-4), 593-600 (2005-05-17)
The stability of baicalin in buffered aqueous solutions at different pHs and in biological fluids, including plasma, urine and tissue homogenates, were investigated in vitro. Structures of the degradation products of baicalin were elucidated by liquid chromatography-electrospray ion trap mass
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实验方案

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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