This product is not a sterile preparation. Stock solutions may be filter sterilized. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/425/i5879pis-ms.pdf
I5879
3-异丁基-1-甲基黄嘌呤
≥99% (HPLC), cAMP and cGMP phosphodiesterases inhibitor, powder
3-Isobutyl-1-methylxanthine
1 of 1 reviewers received a sample product or took part in a promotion
别名:
1-甲基-3-异丁基黄嘌呤, 3,7-二氢-1-甲基-3-(2-甲基丙基)-1H-嘌呤-2,6-二酮, 3-异丁基-1-甲基-2,6(1H,3H)-嘌呤二酮, IBMX
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关于此项目
经验公式（希尔记法）:
C10H14N4O2
化学文摘社编号:
分子量:
222.24
Beilstein:
247859
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
PubChem物質ID:
NACRES:
NA.77
跳至
产品名称
3-异丁基-1-甲基黄嘌呤, ≥99% (HPLC), powder
化驗
≥99% (HPLC)
形狀
powder
顏色
white to off-white
mp
200-201 °C (lit.)
溶解度
DMSO: 1 M (with gentle warming)
ethanol: 10 mg/mL
儲存溫度
−20°C
SMILES 字串
CC(C)CN1C(=O)N(C)C(=O)c2[nH]cnc12
InChI
1S/C10H14N4O2/c1-6(2)4-14-8-7(11-5-12-8)9(15)13(3)10(14)16/h5-6H,4H2,1-3H3,(H,11,12)
InChI 密鑰
APIXJSLKIYYUKG-UHFFFAOYSA-N
基因資訊
human ... PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577)
rat ... Adora1(29290), Adora2a(25369)
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相关类别
1 of 4
此商品 | I7018 | SAB1406296 | 705357 |
|---|---|---|---|
| assay ≥99% (HPLC) | assay ≥99% | assay - | assay 98% (CP) |
| Gene Information human ... PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577) | Gene Information rat ... Adora1(29290), Adora2a(25369) | Gene Information human ... PRKAR1B(5575) | Gene Information - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form powder | form - | form buffered aqueous solution | form powder |
| solubility DMSO: 1 M (with gentle warming), ethanol: 10 mg/mL | solubility DMSO: soluble 1 M (with gentle warming) | solubility - | solubility - |
| color white to off-white | color - | color - | color - |
一般說明
應用
生化／生理作用
由IBMX对磷酸二酯酶的抑制而引起的cAMP水平的增加可激活PKA，而引起增殖减少、分化增加并诱导细胞凋亡。IBMX可抑制苯肾上腺素诱导的气道粘膜神经内分泌上皮细胞5-羟色胺释放。（IC50：1.3 μM）。IBMX还可作为一种腺苷受体拮抗剂。其已表现出对神经肌肉接头、GH3细胞和血管平滑肌细胞中离子通道的抑制作用。IBMX可诱导感觉神经元释放储存在细胞内的钙。
由IBMX对磷酸二酯酶的抑制而引起的cAMP水平的增加可激活PKA，而引起增殖减少、分化增加并诱导细胞凋亡。IBMX可抑制苯肾上腺素诱导的气道粘膜神经内分泌上皮细胞5-羟色胺释放（IC50：1.3 μM）。IBMX还可作为一种腺苷受体拮抗剂。其已表现出对神经肌肉接头、GH3细胞和血管平滑肌细胞中离子通道的抑制作用。IBMX可诱导感觉神经元释放储存在细胞内的钙。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Skin pigmentation enhancers
Comprehensive Series in Photosciences, 3, 637-675 (2001)
Crystal structures of phosphodiesterases 4 and 5 in complex with inhibitor 3-isobutyl-1-methylxanthine suggest a conformation determinant of inhibitor selectivity
Huai Q, et al.
The Journal of Biological Sciences, 279(13), 13095-13101 (2004)
ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos
De L, et al.
Scientific reports, 4, 4898-4898 (2014)
A conserved aromatic lock for the tryptophan rotameric switch in TM-VI of seven-transmembrane receptors
Holst B, et al.
The Journal of Biological Chemistry, 285(6), 3973-3985 (2010)
Culturing INS-1 cells on CDPGYIGSR-, RGD-and fibronectin surfaces improves insulin secretion and cell proliferation.
Kuehn C, et al.
Acta Biomaterialia, 8(2), 619-626 (2012)
实验方案
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
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Is this product sterile?
1 answer-
Helpful?
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After dissolving 3-Isobutyl-1-méthylxanthine (I5879) in DMSO or in pre-warmed methanol, where should it be stored (long term or short term)?
1 answer-
Solutions in DMSO may be stored at -20 °C. The stability of this material in methanol has not been investigated. However, an external publication reports storage of IBMX solutions in ethanol at 0.125 mg/mL at 4 °C for at least three months. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/425/i5879pis-ms.pdfHelpful?
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HPにはDMSOへの溶解度は1 Mと記載されています。 https://www.sigmaaldrich.com/JP/ja/product/sigma/i5879#product-documentation 上記HPからダウンロードしたデータシートにはDMSOへの溶解度は200 mMと記載されています。 メーカー推奨の溶解度はどちらになりますでしょうか。
1 answer-
This material is soluble at a maximum concentration of 1M in DMSO. The 200 mM concentration is cited in Plouffe, B. et al., Methods Enzymol., 484, 295-328 (2010).
This concentration is sufficient for most applications. The product is tested for solubility in pre-warmed methanol at 50 mg/mL. The preferred solvent and stock concentration is dependent upon the application and requirements of the end user. Please see the link below to review additional information provided in the product information sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/425/i5879pis-ms.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the expiration date of Product No. I5879, 3-Isobutyl-1-methylxanthine?
1 answer-
We do not assign an expiration date to this product. We will guarantee the quality of the product for one year from the time of shipment, providing all storage conditions are followed.
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What is the difference between 3-Isobutyl-1-methylxanthine Product I5879 and I7018?
1 answer-
Product No. I5879 is reagent grade. Product No. I7018, SigmaUltra grade, has been subjected to additional testing for trace metal impurities.
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Is Product No. I5879, 3-Isobutyl-1-methylxanthine, appropriate for cell culture use?
1 answer-
Product No. I5879 is not a cell culture tested product. However, customers may wish to try this product in their cell culture applications.
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