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Merck

L3771

十二烷基硫酸钠

greener alternative

BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

别名:

SDS, 十二烷基硫酸盐 钠盐, 十二烷基硫酸钠, 月桂基硫酸盐 钠盐, 月桂基硫酸钠

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25 G

$146.00

100 G

$226.00

500 G

$781.00

1 KG

$1,170.00

10 KG

$5,380.00

$146.00


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关于此项目

线性分子式:
CH3(CH2)11OSO3Na
化学文摘社编号:
分子量:
288.38
Beilstein:
3599286
EC號碼:
MDL號碼:
分類程式碼代碼:
12161902
eCl@ss:
39093306
PubChem物質ID:
NACRES:
NB.22

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生物源

synthetic

品質等級

等級

Molecular Biology

描述

anionic

產品線

BioReagent

化驗

≥98.5% (GC)

形狀

powder

分子量

micellar 18,000

環保替代產品特色

Catalysis
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

凝集数

62

技術

electrophoresis: suitable

雜質

≤2.0% water (Karl Fischer)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

轉變溫度

cloud point >100 °C

溶解度

water: soluble

密度

1.03 g/cm3 at 20 °C (68 °F)

負離子痕跡

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

正離子痕跡

heavy metals (as Pb): ≤10 ppm

吸收

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

適合性

suitable for electrophoresis

異物活動

DNase, RNase, none detected

環保替代類別

儲存溫度

room temp

SMILES 字串

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI 密鑰

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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436143L4509L6026
suitability

suitable for electrophoresis

suitability

-

suitability

-

suitability

-

form

powder

form

solid

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥98.5% (GC)

assay

≥99.0%

assay

≥98.5% (GC)

assay

≥99.0% (GC)

grade

for molecular biology

grade

ACS reagent

grade

-

grade

-

aggregation number

62

aggregation number

-

aggregation number

62

aggregation number

62

一般說明

十二烷基硫酸钠(SDS)也称月桂基硫酸钠,它是一种阴离子表面活性剂,广泛应用于各种科学应用,特别是分子生物学、生物化学和细胞生物学研究。它的功效来源于两性分子特性,这种特性使其可以同时与疏水和亲水分子相互作用。SDS是一种可以使蛋白质增溶和变性的多功能去污剂,这种特性使其成为蛋白质提取和纯化实验中的基本工具。在SDS-PAGE电泳中,通过破坏蛋白质结构,SDS有助于根据分子量进行蛋白质分离。

除了蛋白质分析以外,SDS的应用还包括脂质的电泳分离,从而使研究人员能够对这些重要的细胞成分进行研究和表征。此外,各种杂交和分子生物学技术中也用到了SDS,这体现了它在各种研究领域中的多功能性。SDS在离子和非离子条件下的功效进一步扩展了其用途,使其适用于各种实验环境和要求。它能够通过结合肽链并产生负电荷来裂解蛋白,从而有效消除了凝胶分离中蛋白质形状这一影响因素,确保了准确的蛋白质尺寸测定。
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is used as a surfactant to enhance catalysis.  Click here for more information.

應用

十二烷基硫酸钠已用于:
  • 染色体免疫沉淀
  • SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)
  • 作为用于免疫印迹的放射免疫沉淀检测缓冲液的一种组分
用于增溶和蛋白质变性,适用于PAGE变性应用。 大多数蛋白质以1.4 g SDS/g 蛋白质的比例结合SDS。 相对于结合的SDS自带的总负电荷,蛋白质固有电荷几乎可忽略不计。 每种蛋白质的质荷比基本相同,且基于分子大小在凝胶中迁移。
阴离子洗涤剂

特點和優勢

  • 用于分子生物学和生物化学研究的多功能表面活性剂
  • 适用于电泳应用
  • 经测试证实重金属污染浓度低, 从而确保其适用于各种应用。

分析報告

针对用于变性聚丙烯酰胺凝胶电泳进行了测试。

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

4.1B - Flammable solid hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

338.0 °F

閃點(°C)

170 °C

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
Shane Stegeman et al.
Endocrine-related cancer, 22(2), 265-276 (2015-02-12)
The oncogene MDM4, also known as MDMX or HDMX, contributes to cancer susceptibility and progression through its capacity to negatively regulate a range of genes with tumour-suppressive functions. As part of a recent genome-wide association study it was determined that
S Bavamian et al.
Molecular psychiatry, 20(5), 573-584 (2015-01-28)
Bipolar disorder (BD) is a heritable neuropsychiatric disorder with largely unknown pathogenesis. Given their prominent role in brain function and disease, we hypothesized that microRNAs (miRNAs) might be of importance for BD. Here we show that levels of miR-34a, which
Satoshi Fukuyama et al.
Nature communications, 6, 6600-6600 (2015-03-26)
Seasonal influenza A viruses cause annual epidemics of respiratory disease; highly pathogenic avian H5N1 and the recently emerged H7N9 viruses cause severe infections in humans, often with fatal outcomes. Although numerous studies have addressed the pathogenicity of influenza viruses, influenza
Tadayoshi Hayata et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(2), 318-329 (2014-08-27)
Transforming growth factor (TGF)-β signaling plays critical roles during skeletal development and its excessive signaling causes genetic diseases of connective tissues including Marfan syndrome and acromelic dysplasia. However, the mechanisms underlying prevention of excessive TGF-β signaling in skeletogenesis remain unclear.

实验方案

本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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相关内容

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

细胞裂解和蛋白质提取方法概述了从洗涤剂溶解到机械破坏的各种技术,以支持研究需要。

Product Information Sheet

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is it okay to use 10% SDS dissolved in water that has been autoclaved?

    1 answer
    1. Sodium dodecyl sulfate may decompose with prolonged heating at 40°C or greater. Autoclaving this material is not recommended. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/475/l3771pis.pdf

      Helpful?

  4. What is the storage temperature for the product?

    1 answer
    1. This product is stored at ambient temperature.

      Helpful?

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