Merck

SEQXE

Sigma-Aldrich

SeqPlex DNA扩增试剂盒

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

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别名:
SeqPlex增强型DNA扩增试剂盒, WGA 试剂盒, 全基因组扩增试剂盒
NACRES:
NA.55

质量水平

technique(s)

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

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WGA2WGA4WGA3
vibrant-m

SEQXE

SeqPlex DNA扩增试剂盒

vibrant-m

WGA3

GenomePlex® WGA再扩增试剂盒

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

面向全基因组扩增(WGA)的 SeqPlex Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序(NGS)。 染色质免疫沉淀(ChIP)或福尔马林固定的石蜡包埋组织样品(FFPE)的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。 该试剂盒是 WGA 产品线的延伸,旨在集成到 Illumina®、SOLiD 或 454 测序工作流程中。

应用

SeqPlex DNA 扩增试剂盒已用于全基因组扩增。

特点和优势

  • 采用随机引物技术扩增片段化DNA,如ChIP或FFPE
  • 有助于对低至100 pg的ChIP DNA进行测序
  • 采用增强型引物,可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
  • 可兼容用于二代测序的Illumina®、SOLiD或454文库制备

其他说明

SEQXE-500RXN 是按需生产。获取更多信息,请发邮件至techserv@sial.com 联系技术服务。

法律信息

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
iCAT is a registered trademark of University of Washington

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书

  • Library Preparation Buffer

推荐

产品编号
说明
价格

自备

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
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实验方案

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统,可实现快速的高通量ChIP检测

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