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W4502

Water

Nuclease-Free Water, for Molecular Biology

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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เลือกขนาด

เปลี่ยนการดู
ขนาด/SKUความพร้อมจำหน่ายราคา

เกี่ยวกับสินค้านี้

สูตรเชิงเส้น:
H2O
CASE番号:
น้ำหนักโมเลกุล:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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vapor density

<1 (vs air)

Quality Segment

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable, electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

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ดูการเปรียบเทียบแบบเต็ม

แสดงข้อแตกต่าง

1 of 1

สินค้าชิ้นนี้
95280W350095284
Water Nuclease-Free Water, for Molecular Biology

Sigma-Aldrich

W4502

Water

Water tested according to Ph. Eur.

Sigma-Aldrich

95280

Water

Water sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

W3500

Water

Water Molecular Biology, sterile filtered

Sigma-Aldrich

95284

Water

description

Nuclease-Free Water, for Molecular Biology

description

tested according to Ph. Eur.

description

sterile-filtered, BioReagent, suitable for cell culture

description

Molecular Biology, sterile filtered

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

100

technique(s)

electrophoresis: suitable, PCR: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

FISH: suitable

form

liquid

form

liquid

form

liquid

form

liquid

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

-

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)


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สำรวจสินค้าทั้งหมดของเราภายใต้ Water


คลาสการจัดเก็บ

12 - Non Combustible Liquids

จุดติดไฟ (°F)

Not applicable

จุดวาบไฟ (°C)

Not applicable

อุปกรณ์ป้องกันส่วนบุคคล (PPE)

Eyeshields, Gloves



เลือกจากเวอร์ชันล่าสุด:

ใบรับรองผลการวิเคราะห์ (COA)

หมายเลขล็อต/ชุด

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Questions

1–7 of 7 Questions  
  1. Is this really nuclease-free water?

    1 answer
    1. This product is nuclease-free water. The COA documents all testing performed on this water.

      Helpful?

  2. Es libre de endotoxinas bacterianas?

    1 answer
    1. This product is not endotoxin tested. Currently, there is no PCR-suitable water products available that include endotoxin testing. Please see the links below to review available endotoxin-tested and endotoxin-free product options:
      W3500
      https://www.sigmaaldrich.com/product/sigma/w3500

      TMS-011
      https://www.sigmaaldrich.com/product/mm/tms011

      Helpful?

  3. W4502 Millipore Water: what is the ohms of this water?

    1 answer
    1. The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.

      Helpful?

  4. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  5. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

      Helpful?

  6. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  7. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 10
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-only review