This product is deionized and distilled during production, but it is not guaranteed to be completely ion-free as ICP analysis is not performed. This product is sterilized by 0.2 µm sterile filtration rather than autoclaving. Sterility testing is performed and confirmed in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/w3500
W3500
Water
sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Cell culture grade water, Ultra-filtered Water
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Available to ship TODAYfromMILWAUKEE | $16.00 |
500 mL | Available to ship TODAYfromMILWAUKEE | $22.00 |
1 L | Check Cart for Availability | $44.40 |
6 x 500 mL | Check Cart for Availability | $125.00 |
6 x 1 L | Estimated to ship onOctober 15, 2026fromMILWAUKEE | $229.00 |
24 x 500 mL | Check Cart for Availability | $459.00 |
About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
Technique(s):
cell culture | mammalian: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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vapor density
<1 (vs air)
Quality Segment
vapor pressure
3 mmHg
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mL endotoxin
refractive index
n
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
This product is a very low endotoxin (less than 1 EU/mL), 0.2 micron filtered.
Application
For use in the preparation of cell culture media, and cell suspension and washing solutions.
Other Notes
Easily compare specifications for Water products with the Water specification table
1 of 1
This Item | |||
|---|---|---|---|
| description sterile-filtered, BioReagent, suitable for cell culture | description OmniPur® Grade, Sterile Purified, WFI Quality, Cell Culture Tested | description OmniPur® Grade, Sterile, Nuclease Free | description OmniPur® Grade, DEPC Treated, Autoclaved, Nuclease-Free |
| Quality Level 400 | Quality Level 300 | Quality Level 100 | Quality Level 100 |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| form liquid | form liquid | form liquid | form liquid |
| vapor pressure 3 mmHg | vapor pressure - | vapor pressure - | vapor pressure - |
| refractive index n | refractive index n | refractive index n | refractive index n |
| impurities ≤1 EU/mL endotoxin | impurities - | impurities - | impurities - |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Is it deionized? How is it sterilized?
1 answer-
Helpful?
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Is this product deionized?
1 answer-
This product is deionized and distilled during production. However, it is not subjected to ICP analysis, so it is not warranted to be ion-free.
Helpful?
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How is this product used?
1 answer-
This product is tested for use in cell culture. Parameters such as cytotoxicity, pH, and endotoxin are measured. It may be used for any general application but is of most interest to customers doing cell culture work. It has not been tested for RNAse, DNAse, or protease activity.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/W3500/RNBL9398Helpful?
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