SeqPlex DNA扩增试剂盒

Name: SeqPlex DNA扩增试剂盒
Brand: SIGMA

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

同義詞:

SeqPlex增强型DNA扩增试剂盒, WGA 试剂盒, 全基因组扩增试剂盒

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NACRES:

NA.55

UNSPSC Code:

41121800

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屬性

Quality Segment

200

technique(s)

whole genome amplification: suitable

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

描述

General description

面向全基因组扩增（WGA）的 SeqPlex^™ Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序（NGS）。染色质免疫沉淀（ChIP）或福尔马林固定的石蜡包埋组织样品（FFPE）的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。该试剂盒是 WGA 产品线的延伸，旨在集成到 Illumina^®、SOLiD^™ 或 454 测序工作流程中。

Application

SeqPlex^™ DNA 扩增试剂盒已用于全基因组扩增。

Features and Benefits

随机引物技术可扩增片段化的 DNA，如 ChIP 或 FFPE
便于从低至 100 pg 的 ChIP DNA 进行测序
增强引物，可实现完整的基因组覆盖、最小的序列偏差、引物去除，扩增子大小非常适合新一代测序
与Illumina^®、SOLiD^™或454文库制备兼容，用于新一代测序

Other Notes

SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至techserv@sial.com 联系技术服务。

Legal Information

Illumina is a registered trademark of Illumina, Inc.

SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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本產品	SEQR	WGA2	WGA4
Sigma-Aldrich SEQXE SeqPlex DNA扩增试剂盒	Sigma-Aldrich SEQR SeqPlex RNA扩增试剂盒	Sigma-Aldrich WGA2 GenomePlex^®完整全基因组扩增（WGA）试剂盒	Sigma-Aldrich WGA4 GenomePlex^® 单细胞全基因组扩增试剂盒
description For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.	description For use with high throughput sequencing technologies	description Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue	description Amplify genome of a single cell
technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable
dilution (WGA)	dilution (WTA)	dilution (WGA)	dilution (WGA)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
input purified DNA	input purified RNA	input purified DNA	input purified DNA