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GeneJuice® Transfection Reagent

Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

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Synonym(s):
DNA delivery, Gene delivery

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

transfection: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
7125971281XTG9-RO
technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Roche

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

-

General description

""I use GeneJuice because it′s ease-of-use, low toxicity and wide range of target cells""
Stuart Rulten. Research Fellow. University of Sussex

""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany

""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland

Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.


Cell lines transfected with GeneJuice® Transfection Reagent
Cell Lines:Primary Cells:
10T1/2

293T

3T3 NIH

3T3 Swiss

3T3-L1

A204

A431

A549

alpha TC1-6

AR 42J

As4.1

AtT-20

B50

BC-1

BC-2

BC3

BCBL

BHK-21

C3H/10T1/2

C6

C2C12
Caco-2

Caki-1

Calpan-1

Calu-1

Calu-6

CCL-131

CFPAC-1

Chang Liver

CHO

CHO-7

CHO-IR

CHO-K1

COS-1

COS-7

CS-1

CV-1

Daudi

DDTI MF-2

DT40

ECV304

EL4
ES-E14TG2a

EVSCC17M

H9c2

HCT-116

HEK293

HeLa

HeLa B

HeLa T4

Hep 3B2.1-7

HepG2

Hepa 1-6

Ht-29

HTB-37

HTB-45

Huh-7

HUVEC

IC21

IEC-6

JEG-3

Jurkat

KB
L57-3-11

L-6

L-929

MA-10

McA-RH7777

MCF-7

MCF-10-2A

MDCK

Melanocyte

MG-63

Neuro 2A

Neuroblastoma

NRK

NT2/D1

OV-1063

OVCAR3

P4

P19

PC12

PA317

PAM212
PS-1

R2C

RAW 264.7

RBL-2H3

RMP-41

SAOS-2

SC-1

Schneider line2

SK-N-MC

SK-N-SH

SKOV3

STO

SW-480

SW-837

T3M4

TM4

U937

UCD

Vero

Aortic smooth muscle cells

Astrocytes

Angioblasts

Chondrocytes

Chromaffin cells

Epithelial cells:

mammary

prostate

tracheal

Fibroblasts

Keratinocytes

Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

Features and Benefits

  • Highly efficient DNA transfer for both stable and transient transfections
  • Compatibility with both serum-containing and serum-free media
  • Simple protocolno need for media changes
  • Ideal for high-throughput transfection in a multi-well plate format
  • Ideal for retrovirus production for T-cell transduction
  • Works for a multitude of cell types with minimal optimization
  • Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers
  • The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates

Warning

Toxicity: Flammable (J)

Preparation Note

For protocol, please click (here)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

GeneJuice® is a registered trademark of EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

67.1 °F - Information taken from reference works and the literature.

Flash Point(C)

19.5 °C - Information taken from reference works and the literature.


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Sharon Eisenberg et al.
Molecular and cellular biology, 31(19), 3938-3952 (2011-08-03)
The trafficking, membrane localization, and lipid raft association of Ras proteins, which are crucial oncogenic mediators, dictate their isoform-specific biological responses. Accordingly, their spatiotemporal dynamics are tightly regulated. While extensively studied for H- and K-Ras, such information on N-Ras, an
Linda Brunotte et al.
The Journal of biological chemistry, 286(44), 38748-38756 (2011-09-16)
The nucleoprotein (NP) of Lassa virus (LASV) strain AV was expressed in a recombinant baculovirus system. The crystal structure of full-length NP was solved at a resolution of 2.45 Å. The overall fold corresponds to that of NP of LASV
David D New et al.
American journal of physiology. Cell physiology, 302(1), C122-C130 (2011-09-24)
Extracellular matrix accumulation contributes to the progression of chronic kidney disease. Many growth factors including insulin-like growth factor-I (IGF-I) enhance matrix protein accumulation. Proximal tubular epithelial cells (PTCs) synthesize matrix proteins. NADPH oxidases are major sources of reactive oxygen species
Natasha C Lucki et al.
The Journal of biological chemistry, 286(22), 19399-19409 (2011-04-16)
Sphingolipid metabolites, such as ceramide (Cer), sphingosine (SPH), and sphingosine 1-phosphate (S1P), contribute to multiple aspects of carcinogenesis including cell proliferation, migration, angiogenesis, and tumor resistance. The cellular balance between Cer and S1P levels, for example, is an important determinant
Sandra Habbig et al.
The Journal of cell biology, 193(4), 633-642 (2011-05-11)
The conserved Hippo signaling pathway regulates organ size in Drosophila melanogaster and mammals and has an essential role in tumor suppression and the control of cell proliferation. Recent studies identified activators of Hippo signaling, but antagonists of the pathway have

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