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MilliporeSigma

AP130P

Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate

0.8 mg/mL, Chemicon®

Synonym(s):

Goat Anti-Mouse IgG Antibody

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2 ML

$268.00

$268.00


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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702

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Product Name

Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate, 0.8 mg/mL, Chemicon®

biological source

goat

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

0.8 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

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This Item
AP160PAP113PAP181P
biological source

goat

biological source

rabbit

biological source

goat

biological source

goat

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

species reactivity

mouse

species reactivity

mouse

species reactivity

human

species reactivity

mouse

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Application

Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate has been used in western blotting[1](1:400) and immunofluorescence (1:500)[2]

Biochem/physiol Actions

IgG facilitates antibody-dependent cell-mediated cytotoxicity (ADCC). It also aids in the activation of the classical pathway of the complement system. IgG neutralizes virus particles and toxins. IgM, an antibody that is expressed early during an immune response, aids in engulfing apoptotic cells. It exhibits polyreactive and autoreactive functionality. IgM induces tissue homeostasis by mediating the clearance of tissue-based molecules. Lower levels of IgM in serum are associated with increased autoimmune response and a higher risk of developing infections.

General description

Immunoglobulin G (IgG) is the most abundant immunoglobulin in the human serum. IgM constitutes 5-10% of total serum immunoglobulins. These immunoglobulins comprise four polypeptide chains with two heavy (H) chains and two light (L) chains. These polypeptide chains are inter-linked by disulfide bonds. IgM is a highly conserved antibody in vertebrates. IgM exists as a pentamer. Both IgG and IgM are secreted by B cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Obesity (Silver Spring, Md.), 27(1), 103-111 (2018-12-07)
Naringenin, a citrus flavonoid, prevents diet-induced weight gain and improves glucose and lipid metabolism in rodents. There is evidence that naringenin activates brown fat and increases energy expenditure in mice, but little is known about its effects in humans. The
Mari H Savolainen et al.
Neurobiology of disease, 68, 1-15 (2014-04-22)
The misfolding and aggregation of α-synuclein (aSyn) eventually lead to an accumulation of toxic forms that disturb normal neuronal function and result in cell death. aSyn rich inclusions are seen in Parkinson's disease, dementia with Lewy bodies and other synucleinopathies.
Dusan Bencina et al.
Avian pathology : journal of the W.V.P.A, 34(6), 463-472 (2006-03-16)
Maternal antibodies can protect avian embryos against vertically transmitted pathogens during embryogenesis and also young birds after hatching. In contrast to the well-known transfer of maternal immunoglobulin (Ig) G (also termed IgY) from the yolk to embryonic blood, information about

Questions

  1. What do you recommend to reconstitute AP130P? We will be using this for ELISA

    1 answer
    1. This product can be reconstituted with sterile distilled water to match the volume indicated on the vial label.

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