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IP05

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Synonym(s):

Affinity resin, Protein G/Agarose

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UNSPSC Code:
41116133
NACRES:
NA.56

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

protein purification: suitable

suitability

suitable for microbiology

shipped in

wet ice

storage temp.

2-8°C

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This Item
IP04IP02IP10
Protein G Plus/Protein A Agarose Suspension Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Millipore

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G Plus-Agarose Suspension Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Millipore

IP04

Protein G Plus-Agarose Suspension

Protein A Agarose Suspension Protein A Agarose Suspension designed for immunoprecipitation applications

Millipore

IP02

Protein A Agarose Suspension

Protein G Plus/Protein A-Agarose A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.

Millipore

IP10

Protein G Plus/Protein A-Agarose

technique(s)

protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

protein purification: suitable

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

suitability

suitable for microbiology

suitability

suitable for immunoprecipitation

suitability

-

suitability

suitable for microbiology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Application

Immunoprecipitation (see comments)

Physical form

33% slurry in PBS.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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