Collagenase from C. histolyticum
is widely used for the disaggregation of many types of tissues (e.g.
, lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors, umbilical cord) and for the preparation of single-cell suspensions for the establishment of primary cell culture systems. Collagenase A is recommended when yield, viability and functionality are important.Clostridium
collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc
. However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically.