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Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities.

Journal of agricultural and food chemistry (2011-08-13)
Xiu-Juan Ye, Tzi-Bun Ng, Zu-Jian Wu, Lian-Hui Xie, Evandro-Fei Fang, Jack-Ho Wong, Wen-Liang Pan, Sze-Stephen-Cho Wing, Yan-Bo Zhang
ABSTRACT

A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50=5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50=96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50=53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50=50 and 90 μM, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mono S® 5/50 GL, 10 μm particle size, L × I.D. 5 cm × 5 mm

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