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BICD1 mediates HIF1α nuclear translocation in mesenchymal stem cells during hypoxia adaptation.

Cell death and differentiation (2018-11-23)
Hyun Jik Lee, Young Hyun Jung, Ji Young Oh, Gee Euhn Choi, Chang Woo Chae, Jun Sung Kim, Jae Ryong Lim, Seo Yihl Kim, Sei-Jung Lee, Je Kyung Seong, Ho Jae Han

Hypoxia inducible factor 1α (HIF1α) is a master regulator leading to metabolic adaptation, an essential physiological process to maintain the survival of stem cells under hypoxia. However, it is poorly understood how HIF1α translocates into the nucleus in stem cells under hypoxia. Here, we investigated the role of a motor adaptor protein Bicaudal D homolog 1 (BICD1) in dynein-mediated HIF1α nuclear translocation and the effect of BICD1 regulation on hypoxia adaptation and its therapeutic potential on human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). In our results, silencing of BICD1 but not BICD2 abolished HIF1α nuclear translocation and its activity. BICD1 overexpression further enhanced hypoxia-induced HIF1α nuclear translocation. Hypoxia stimulated direct bindings of HIF1α to BICD1 and the intermediate chain of dynein (Dynein IC), which was abolished by BICD1 silencing. Akt inhibition reduced the binding of BICD1 to HIF1α and nuclear translocation of HIF1α. Conversely, Akt activation or GSK3β silencing further enhanced the hypoxia-induced HIF1α nuclear translocation. Furthermore, BICD1 silencing abolished hypoxia-induced glycolytic reprogramming and increased mitochondrial ROS accumulation and apoptosis in UCB-MSCs under hypoxia. In the mouse skin wound healing model, the transplanted cell survival and skin wound healing capacities of hypoxia-pretreated UCB-MSCs were reduced by BICD1 silencing and further increased by GSK3β silencing. In conclusion, we demonstrated that BICD1-induced HIF1α nuclear translocation is critical for hypoxia adaptation, which determines the regenerative potential of UCB-MSCs.

Product Number
Product Description

Duolink® In Situ Detection Reagents Red
MG-132, Ready Made Solution, ≥90% (HPLC)
Nocodazole, ≥99% (TLC), powder
Trypan Blue, powder, BioReagent, suitable for cell culture
Duolink® In Situ PLA® Probe Anti-Mouse PLUS
Duolink® In Situ PLA® Probe Anti-Rabbit MINUS
Wortmannin, from Penicillium funiculosum, ≥98% (HPLC and TLC)
Tetramethylrhodamine ethyl ester perchlorate, suitable for fluorescence, ≥90% (HPCE)
SC79, ≥97% (NMR)

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