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  • Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Methods in molecular biology (Clifton, N.J.) (2018-04-02)
Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen
ABSTRACT

In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis.

MATERIALS
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Product Description

Sigma-Aldrich
DLAM-AβVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
TLAM-Iδ1MεTγ-13CH3 Methyl Labeling Kit