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  • An ultrasensitive platform for PCB77 detection: New strategy for liquid crystal-based aptasensor fabrication.

An ultrasensitive platform for PCB77 detection: New strategy for liquid crystal-based aptasensor fabrication.

Journal of hazardous materials (2020-07-30)
Asma Verdian, Zeinab Rouhbakhsh, Ebrahim Fooladi
ABSTRACT

Polychlorinated biphenyls (PCBs) are considered persistent bio-accumulative toxicants which threats global food safety and environmental health. Traditional analytical techniques for detection of PCBs are time-consuming and they do not satisfy urgent need for rapid and accurate monitoring of these persistent pollutants. Biosensor technology may be promising in this respect. Here we demonstrate a novel liquid crystal (LC)-based aptasensing platform as a promising label-free and rapid biosensor for PCB77 detection. This novel molecular strategy utilize triple-helix molecular conformational switch which is mediated formation of duplex on sensing platform in presence of target. Duplex forming leads to optical change from dark to bright in a liquid crystal based aptasensor. The limit of quantification of the LC-aptasensor to PCB77 is 1.5 × 10-5 μg/L with comparable selectivity. Besides, we also demonstrated that this system is able to detect PCB77 in tap water, environmental water and milk. This strategy has potential for label-free and portable detection of different targets without any aptamer sequence length restrictions.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzyl butyl phthalate, 98%
Sigma-Aldrich
4′-Pentyl-4-biphenylcarbonitrile, liquid crystal (nematic), 98%