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Cultured 3T3L1 adipocytes dispose of excess medium glucose as lactate under abundant oxygen availability.

Scientific reports (2014-01-15)
David Sabater, Sofía Arriarán, María del Mar Romero, Silvia Agnelli, Xavier Remesar, José Antonio Fernández-López, Marià Alemany
ABSTRACT

White adipose tissue (WAT) produces lactate in significant amount from circulating glucose, especially in obesity;Under normoxia, 3T3L1 cells secrete large quantities of lactate to the medium, again at the expense of glucose and proportionally to its levels. Most of the glucose was converted to lactate with only part of it being used to synthesize fat. Cultured adipocytes were largely anaerobic, but this was not a Warburg-like process. It is speculated that the massive production of lactate, is a process of defense of the adipocyte, used to dispose of excess glucose. This way, the adipocyte exports glucose carbon (and reduces the problem of excess substrate availability) to the liver, but the process may be also a mechanism of short-term control of hyperglycemia. The in vivo data obtained from adipose tissue of male rats agree with this interpretation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
GenElute Mammalian Total RNA Miniprep Kit, sufficient for 70 purifications
Sigma-Aldrich
GenElute Mammalian Total RNA Miniprep Kit, sufficient for 350 purifications
Sigma-Aldrich
GenElute Mammalian Total RNA Miniprep Kit, sufficient for 10 purifications