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Nanopatterned cadmium selenide Langmuir-Blodgett platform for leukemia detection.

Analytical chemistry (2012-03-03)
Aditya Sharma, Chandra M Pandey, Zimple Matharu, Udit Soni, Sameer Sapra, Gajjala Sumana, Manoj K Pandey, Tathagat Chatterjee, Bansi D Malhotra
ABSTRACT

We present results of the studies relating to preparation of Langmuir-Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium-tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air-water interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10(-6) to 10(-14) M within 120 s, has a shelf life of 2 months, and can be used about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trioctylphosphine oxide, ReagentPlus®, 99%
Sigma-Aldrich
Trioctylphosphine oxide, technical grade, 90%
Sigma-Aldrich
Trioctylphosphine oxide, for extraction analysis, ≥98.5%

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