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Acaricide resistance status in Indian isolates of Hyalomma anatolicum.

Experimental & applied acarology (2012-07-05)
K P Shyma, Sachin Kumar, Anil Kumar Sharma, D D Ray, S Ghosh
ABSTRACT

The multi host tick, Hyalomma anatolicum, is the commonest Hyalomma species in India and cattle serves as the main host of this species. A study to evaluate the acaricide resistance of H. anatolicum to deltamethrin, cypermethrin and diazinon was conducted in 20 areas located in three agro climatic regions known to have abundance of the species. Results obtained by the "larval packet test" (LPT) showed a low grade resistance (level-I, RF <5) in the tick species to both deltamethrin and cypermethrin in 10 areas and higher grade resistance (level-II, RF <25) to deltamethrin in one area, where intensive use of synthetic pyrethroids are practiced for tick control. Low grade resistance to diazinon (level I) was recorded in six areas where organophosphates compounds are extensively used for agricultural practices allowing increased exposure of the moulting instars of the ticks to these chemicals. Biochemical analysis of the samples suggested involvement of esterase and alterations of acetylcholinesterase in the resistance mechanisms.

MATERIALS
Product Number
Brand
Product Description

Supelco
Diazinon solution, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard
Supelco
Diazinon, PESTANAL®, analytical standard
Supelco
Diazinon, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland