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  • Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives.

Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives.

Biotechnology letters (2012-12-18)
Jung-Hee Woo, Tae-Hyung Kwon, Jun-Tae Kim, Choong-Gon Kim, Eun Yeol Lee
ABSTRACT

A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its derivatives, such as 2,3- and 4-chlorostyrene oxides, as a sole carbon source. Gordonia sp. H37 exhibited high enantioselective hydrolysis activity for 4-chlorostyrene oxide with an enantiomeric ratio of 27. Gordonia sp. H37 preferentially hydrolyzed the (R)-enantiomer of styrene oxide derivatives resulting in the preparation of a (S)-enantiomer with enantiomeric excess greater than 99.9 %. The enantioselective EHase activity was identified and characterized in various PAH-degrading bacteria, and whole cell Gordonia sp. H37 was employed as a biocatalyst for preparing enantiopure (S)-styrene oxide derivatives.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(R)-(+)-Styrene oxide, 97%, optical purity ee: 97% (GLC)
Sigma-Aldrich
(R)-(+)-Styrene oxide, ChiPros®, produced by BASF, ≥98%
Sigma-Aldrich
Styrene oxide, 97%
Sigma-Aldrich
(S)-(−)-Styrene oxide, 98%, optical purity ee: 98% (GC)