• Home
  • Search Results
  • Comparison of quantitative performance of three fluorescence labels in CE/LIF analysis of aspartate and glutamate in brain microdialysate.

Comparison of quantitative performance of three fluorescence labels in CE/LIF analysis of aspartate and glutamate in brain microdialysate.

Electrophoresis (2011-10-20)
Zsolt Wagner, Tamás Tábi, Gergely Zachar, András Csillag, Eva Szöko
ABSTRACT

Three different fluorescent tags have been compared for the quantitative analysis of aspartate and glutamate in brain microdialysate samples. Separation conditions have been optimized to achieve short analysis time using reversed polarity separation in coated capillary. Method validation has revealed similar quantification limit of 0.1 μM of analytes using either of the labels, although LOD values were different: 7.8-9.8 nM for 4-fluoro-7-nitro-2,1,3-benzoxadiazole, 3.5 nM for fluorescein-5-isothiocyanate and 1.3-1.5 nM for carboxyfluorescein succinimidyl ester derivatives. The almost two orders of magnitude difference between LOD and LOQ values is likely due to the unreliable derivatization reaction at low sample concentration. Based on the superior stability, FITC derivatization was used for the analysis of biological samples. The applicability of the method has been demonstrated by analyzing basal and potassium evoked amino acid concentrations in individual brain microdialysate samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Fluoro-7-nitrobenzofurazan, ≥98% (elemental analysis)
Sigma-Aldrich
4-Fluoro-7-nitrobenzofurazan, BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.