• Home
  • Search Results
  • Asymmetric reduction of o-chloroacetophenone with Candida pseudotropicalis 104.

Asymmetric reduction of o-chloroacetophenone with Candida pseudotropicalis 104.

Biotechnology progress (2006-10-07)
Qing Xie, Jianping Wu, Gang Xu, Lirong Yang
ABSTRACT

The asymmetric reduction of o-chloroacetophenone 1 with Candida pseudotropicalis 104 produced the corresponding (S)-1-(2-chloro-phenyl)-ethanol 2 with the enantiomeric excess (ee >99%) without addition of any cosolvent. The cell could tolerate high ketone 1 concentration of 233.8 mmol/L (i.e., 36 g/L) with considerable reduction activity in this method. The product 2 concentration achieved 38.9 and 58.4 mmol/L with cells of 40 and 60 g(DCW) (dry cell weight)/L, respectively, in 24 h. The optimum reaction time, the effect of substrate concentration, cosubstrate type and concentration, and cell concentration in the reaction were investigated in this paper.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Chloroacetophenone, 98%
Sigma-Aldrich
2-Chloroacetophenone, purum, ≥98.0% (GC)