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  • A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis.

A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis.

Talanta (2011-05-03)
Fan Yang, Xin-chun Li, Wen Zhang, Jian-bin Pan, Zuan-guang Chen
ABSTRACT

In this paper, a compact and inexpensive light emitting diode induced fluorescence (LED-IF) detector with simplified optical configuration was developed and assembled in an integrated microfluidic device for microscale electrophoresis. The facile detector mainly consisted of an LED, a focusing pinhole, an emission filter and a photodiode, and was encapsulated in the upper layer of an aluminum alloy device with two layers. At the bottom layer, integrated circuit (IC) was assembled to manipulate the voltage for sample injection and separation, LED emission and signal amplifying. A high-power LED with fan-shaped heat sink was used as excitation source. The excitation light was focused by a 1.1mm diameter pinhole fabricated in a thin piece of silver foil, and the obtained sensitivity was about 3 times as high as that using electrode plate. Other important parameters including LED driven current, fluorescence collection angle and detection distance have also been investigated. Under optimal conditions, considerable high-response of 0.09 fmol and 0.18 fmol mass detection limits at 0.37 nL injection volume for sodium fluorescein (SF) and FITC was achieved, respectively. This device has been successfully employed to separate penicillamine (PA) enantiomers. Due to such significant features as low-cost, integration, miniaturization, and ease of commercialization, the presented microfluidic device may hold great promise for clinical diagnostics and bioanalytical applications.

MATERIALS
Product Number
Brand
Product Description

Silver, foil, 0.5m coil, thickness 0.15mm, as rolled, 99.95+%
Silver, foil, 10mm disks, thickness 0.125mm, as rolled, 99.99+%
Silver, foil, 10mm disks, thickness 0.008mm, 99.95+%
Silver, foil, 10mm disks, thickness 0.05mm, as rolled, 99.95+%
Silver, foil, 10mm disks, thickness 0.25mm, as rolled, 99.99+%
Silver, foil, 10mm disks, thickness 0.008mm, 99.97%
Silver, foil, 15mm disks, thickness 0.003mm, 99.9%
Silver, foil, 10mm disks, thickness 1.0mm, as rolled, 99.99+%
Silver, foil, light tested, 50x50mm, thickness 0.0125mm, 99.95+%
Silver, foil, not light tested, 100x100mm, thickness 0.0025mm, 99.97%
Silver, foil, 6mm disks, thickness 0.015mm, 99.97%
Silver, foil, 6mm disks, thickness 0.125mm, annealed, 99.95+%
Silver, foil, 8mm disks, thickness 0.007mm, 99.97%
Silver, foil, 8mm disks, thickness 0.035mm, as rolled, 99.95+%
Silver, foil, light tested, 100x100mm, thickness 0.025mm, as rolled, 99.99+%
Silver, foil, light tested, 150x150mm, thickness 0.05mm, annealed, 99.95+%
Silver, foil, light tested, 50x50mm, thickness 0.015mm, 99.97%
Silver, foil, 8mm disks, thickness 0.25mm, as rolled, 99.95+%
Silver, foil, 8mm disks, thickness 0.008mm, 99.95+%
Silver, foil, not light tested, 100x100mm, thickness 0.003mm, 99.9%
Silver, foil, 6mm disks, thickness 0.5mm, as rolled, 99.99+%
Silver, foil, light tested, 150x150mm, thickness 0.05mm, as rolled, 99.95+%
Silver, foil, not light tested, 150x150mm, thickness 0.025mm, as rolled, 99.95+%
Silver, foil, 8mm disks, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, 6mm disks, thickness 0.5mm, as rolled, 99.95+%
Silver, foil, 6mm disks, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, light tested, 150x150mm, thickness 0.025mm, as rolled, 99.95+%
Silver, foil, not light tested, 25x25mm, thickness 0.006mm, 99.97%
Silver, foil, 8mm disks, thickness 0.5mm, as rolled, 99.95+%
Silver, foil, 8mm disks, thickness 0.009mm, 99.97%