Myoglobin as a new fluorescence probe to sense H2S.

Protein and peptide letters (2010-09-23)
Maria Strianese, Franco De Martino, Claudio Pellecchia, Giuseppe Ruggiero, Sabato D'Auria
ABSTRACT

A new, fast, simple and cost-effective sensing device for monitoring H(2)S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H(2)S detection are reported. The two different commercial labels Cy3 and Atto620 were selected for this study. A high selectivity for detecting H(2)S against other thiols was found. The applicability of the proposed sensing system was successfully explored not only in solution but also when applied in the form of a solid state device.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 633, BioReagent, suitable for fluorescence
Supelco
Atto 633 NHS ester, BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
Sigma-Aldrich
Atto 633 amine, suitable for fluorescence
Sigma-Aldrich
Atto 633 iodoacetamide, suitable for fluorescence
Sigma-Aldrich
Atto 633 azide, suitable for fluorescence, ≥80.0% (HPCE)

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