• Home
  • Search Results
  • Phospholipid encapsulated semiconducting polymer nanoparticles: their use in cell imaging and protein attachment.

Phospholipid encapsulated semiconducting polymer nanoparticles: their use in cell imaging and protein attachment.

Journal of the American Chemical Society (2010-02-24)
Philip Howes, Mark Green, James Levitt, Klaus Suhling, Mary Hughes
ABSTRACT

Semiconducting polymer nanospheres (SPNs) have been synthesized and encapsulated in phospholipid micelles by a solvent evaporation technique. Four different conjugated polymers were used, yielding aqueous dispersions of nanoparticles which emit across the visible spectrum. The synthesis was simple and easily reproducible, and the resultant nanoparticle solutions exhibited high colloidal stability. As these encapsulated SPNs do not contain any toxic materials and show favorable optical properties, they appear to be a promising imaging agent in biomedical and imaging applications. The SPNs were used in simple fluorescence imaging experiments and showed uptake in SH-SY5Y neuroblastoma and live HeLa cells. Carboxylic acid functionalized SPNs were also synthesized and conjugated to bovine serum albumin (BSA) by carbodiimide-mediated chemistry, a key step in the realization of targeted imaging using conjugated polymers.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 390 azide, suitable for fluorescence
Supelco
Atto 390 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.