RNA self-ligation: from oligonucleotides to full length ribozymes.

Biochimie (2012-04-03)
Slawomir Gwiazda, Kristina Salomon, Bettina Appel, Sabine Müller
ABSTRACT

The RNA-world-theory is one possible explanation of how life on earth has evolved. In this context it is of high interest to search for molecular systems, capable of self-organization into structures with increasing complexity. We have engineered a simple catalytic system in which two short RNA molecules can catalyze their own ligation to form a larger RNA construct. The system is based on the hairpin ribozyme using a 2',3'-cyclophosphate as activated species for ligation. 2',3'-cyclic phosphates can be easily formed and occur in many natural systems, thus being superior candidates for activated building blocks in RNA world scenarios.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 680, BioReagent, suitable for fluorescence, ≥90% (HPLC)
Sigma-Aldrich
Atto 680 NHS ester, BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
Sigma-Aldrich
Atto 680 maleimide, BioReagent, suitable for fluorescence, ≥90% (HPCE)

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