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Twin ribozyme mediated removal of nucleotides from an internal RNA site.

Biochemical and biophysical research communications (2007-09-11)
Irene Drude, Stéphanie Vauléon, Sabine Müller
ABSTRACT

Over the past two decades, the structure and mechanism of catalytic RNA have been extensively studied; now ribozymes are understood well enough to turn them into useful tools. After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides into a predefined position of a suitable substrate RNA, we here show that a similar type of twin ribozyme is also capable of mediating the opposite reaction: the site-specific removal of nucleotides. In particular, we have designed a twin ribozyme that supports the deletion of four uridine residues from a given RNA substrate. This reaction is a kind of RNA recombination that in the specific context of gene therapy mimics, at the level of RNA, the correction of insertion mutations. As a result of the twin ribozyme driven reaction, 17% of substrate are converted into the four nucleotides shorter product RNA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 680, BioReagent, suitable for fluorescence, ≥90% (HPLC)
Sigma-Aldrich
Atto 680 NHS ester, BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
Sigma-Aldrich
Atto 680 maleimide, BioReagent, suitable for fluorescence, ≥90% (HPCE)

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