• Home
  • Search Results
  • Evidence of Phytoalexins in Cucumber Leaves Infected with Powdery Mildew following Treatment with Leaf Extracts of Reynoutria sachalinensis.

Evidence of Phytoalexins in Cucumber Leaves Infected with Powdery Mildew following Treatment with Leaf Extracts of Reynoutria sachalinensis.

Plant physiology (1997-03-01)
F. Daayf, A. Schmitt, R. R. Belanger
ABSTRACT

Phenolic compounds extracted from cucumber (Cucumis sativus L.) leaves were separated and analyzed for their differential presence and fungitoxicity in relation to a prophylactic treatment with Milsana (Compo, Munster, Germany) against powdery mildew (Sphaerotheca fuliginea). Based on our extraction and purification procedures, at least eight separate phenolic compounds with antifungal activity were identified as intrinsic components of cucumber plants. Of these compounds, six displayed a significant increase in concentration as a result of elicitation with Milsana, this being particularly evident when the plant was stressed by the pathogen. The combined amounts of these antifungal compounds in treated plants was nearly five times the level found in control plants. One week after Milsana application, some of the antifungal compounds obtained through hydrolysis of their glycosidic links were also detected in their free form, indicating that they are likely liberated from conjugated phenolics by enzymatic hydrolysis in planta. To our knowledge, these results provide the first direct evidence that cucumber plants produce elevated levels of phytoalexins in response to an eliciting treatment after infection.

MATERIALS
Product Number
Brand
Product Description

Supelco
Silica gel orange, granular, 0.2-1 mm
Supelco
Silica gel, high-purity grade, pore size 60 Å, 2-25 μm particle size, without binder, pore volume 0.75 cm3/g, suitable for thin layer chromatography (TLC)
Supelco
Silica gel, high-purity grade, pore size 60 Å, 5-25 μm particle size, without binder, suitable for thin layer chromatography (TLC)
Supelco
Silica gel, high-purity grade, pore size 60 Å, 2-25 μm particle size, without binder, with fluorescent indicator, pore volume 0.75 cm3/g, suitable for thin layer chromatography (TLC)
Supelco
Silica gel, technical grade (w/ Ca, ~0.1%), 60Å, 230-400 mesh particle size, Ca 0.1-0.3 %

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.