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Metabolic engineering of Corynebacterium glutamicum for cadaverine fermentation.

Bioscience, biotechnology, and biochemistry (2007-09-27)
Takashi Mimitsuka, Hideki Sawai, Masahiro Hatsu, Katsushige Yamada
ABSTRACT

Cadaverine, the expected raw material of polyamides, is produced by decarboxylation of L-lysine. If we could produce cadaverine from the cheapest sugar, and as a renewable resource, it would be an effective solution against global warming, but there has been no attempt to produce cadaverine from glucose by fermentation. We focused on Corynebacterium glutamicum, whose L-lysine fermentation ability is superior, and constructed a metabolically engineered C. glutamicum in which the L-homoserine dehydrogenase gene (hom) was replaced by the L-lysine decarboxylase gene (cadA) of Escherichia coli. In this recombinant strain, cadaverine was produced at a concentration of 2.6 g/l, equivalent to up to 9.1% (molecular yield) of the glucose transformed into cadaverine in neutralizing cultivation. This is the first report of cadaverine fermentation by C. glutamicum.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cadaverine, purum, ≥97.0% (GC)