MilliporeSigma
  • Home
  • Search Results
  • Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.

Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.

The Biochemical journal (1971-02-01)
R Fields, H B Dixon
ABSTRACT

A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is discussed.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,4-Dinitrophenylhydrazine hydrochloric acid solution, ~0.005 M in ethanol, for TLC derivatization