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Statistical optimization of ultraviolet irradiate conditions for vitamin D₂ synthesis in oyster mushrooms (Pleurotus ostreatus) using response surface methodology.

PloS one (2014-04-17)
Wei-Jie Wu, Byung-Yong Ahn
ABSTRACT

Response surface methodology (RSM) was used to determine the optimum vitamin D2 synthesis conditions in oyster mushrooms (Pleurotus ostreatus). Ultraviolet B (UV-B) was selected as the most efficient irradiation source for the preliminary experiment, in addition to the levels of three independent variables, which included ambient temperature (25-45°C), exposure time (40-120 min), and irradiation intensity (0.6-1.2 W/m2). The statistical analysis indicated that, for the range which was studied, irradiation intensity was the most critical factor that affected vitamin D2 synthesis in oyster mushrooms. Under optimal conditions (ambient temperature of 28.16°C, UV-B intensity of 1.14 W/m2, and exposure time of 94.28 min), the experimental vitamin D2 content of 239.67 µg/g (dry weight) was in very good agreement with the predicted value of 245.49 µg/g, which verified the practicability of this strategy. Compared to fresh mushrooms, the lyophilized mushroom powder can synthesize remarkably higher level of vitamin D2 (498.10 µg/g) within much shorter UV-B exposure time (10 min), and thus should receive attention from the food processing industry.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ergocalciferol (Vitamin D2), Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Ergocalciferol, ≥98.0% (sum of enantiomers, HPLC)
Sigma-Aldrich
Ergosterol, ≥75%
Supelco
Ergosterol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Ergocalciferol, 40,000,000 USP units/g
Ergocalciferol, European Pharmacopoeia (EP) Reference Standard
Ergosterol, European Pharmacopoeia (EP) Reference Standard
Supelco
Vitamin D2 solution, 1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
USP
Ergocalciferol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Vitamin D2 solution, 100 μg/mL in ethanol, 98% (CP)