• Home
  • Search Results
  • In vivo immunostaining of hemocyte compartments in Drosophila for live imaging.

In vivo immunostaining of hemocyte compartments in Drosophila for live imaging.

PloS one (2014-06-04)
Gábor Csordás, Gergely I B Varga, Viktor Honti, Ferenc Jankovics, Éva Kurucz, István Andó

In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too.

Product Number
Product Description

DAPI, for nucleic acid staining
Heptane, suitable for HPLC, ≥99%
Heptane, anhydrous, 99%
Heptane, ReagentPlus®, 99%
Dichlorvos, PESTANAL®, analytical standard
Heptane, HPLC Plus, for HPLC, GC, and residue analysis, 99%
Heptane, puriss. p.a., reag. Ph. Eur., ≥99% n-heptane basis (GC)
Heptane, analytical standard
Heptane, Pharmaceutical Secondary Standard; Certified Reference Material
Heptane, suitable for HPLC, ≥96%
Heptane, biotech. grade, ≥99%
Heptane, HPLC grade, ≥99%

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.